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Showing: 801 - 850 of 867 results

  1. pLEX307_CR-PDHA2S291A

    Plasmid
    #115203
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115203-plasmid-map-sequence-id-232429

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  2. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid
    #115187
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115187-plasmid-map-sequence-id-225414

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  3. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid
    #115188
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Expression
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115188-plasmid-map-sequence-id-225424

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  13. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  20. P[acman]-Alpha2

    Plasmid
    #165859
    Purpose
    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165859-plasmid-map-sequence-id-336455

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  21. P[acman]-Omega2

    Plasmid
    #165861
    Purpose
    Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165861-plasmid-map-sequence-id-336788

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  22. P[acman]-Alpha1

    Plasmid
    #165858
    Purpose
    Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165858-plasmid-map-sequence-id-336456

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  23. P[acman]-Omega1

    Plasmid
    #165860
    Purpose
    Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165860-plasmid-map-sequence-id-336787

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  24. pRMC2-msfGFP

    Plasmid
    #194913
    Purpose
    Tetracycline inducible expression of msfGFP in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194913-plasmid-map-sequence-id-384624

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  25. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

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  26. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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  29. pGem-LP1-loxP-bar-lox2272-71

    Plasmid
    #168785
    Purpose
    Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168785-plasmid-map-sequence-id-350000

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  30. pGem-LP2-loxP-bar-lox2272-71

    Plasmid
    #168786
    Purpose
    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168786-plasmid-map-sequence-id-353782

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  32. pC-BAR-YR

    Plasmid
    #61766
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    61766-plasmid-map-sequence-id-442755

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  33. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit
    #1000000143
    Purpose
    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
    Depositor
    Gerard Wright
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  34. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Dominic Esposito
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV.hSyn.CaMPARI.WPRE.SV40 (AAV9)

    Viral Prep
    #100832-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.hSyn.CaMPARI.WPRE.SV40 (#100832). In addition to the viral particles, you will also receive purified pAAV.hSyn.CaMPARI.WPRE.SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger, Eric Schreiter
    Article
    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d
    Promoter
    Syn
    Tags
    None
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  36. pAAV.hSyn.CaMPARI.WPRE.SV40 (AAV1)

    Viral Prep
    #100832-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.CaMPARI.WPRE.SV40 (#100832). In addition to the viral particles, you will also receive purified pAAV.hSyn.CaMPARI.WPRE.SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger, Eric Schreiter
    Article
    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d
    Promoter
    Syn
    Tags
    None
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. pAAV.hSyn.CaMPARI.WPRE.SV40 (AAV5)

    Viral Prep
    #100832-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.hSyn.CaMPARI.WPRE.SV40 (#100832). In addition to the viral particles, you will also receive purified pAAV.hSyn.CaMPARI.WPRE.SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger, Eric Schreiter
    Article
    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d
    Promoter
    Syn
    Tags
    None
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)

    Viral Prep
    #174007-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Mark Histed
    Article
    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22
    Promoter
    Syn
    Tags
    None
    Available since
    May 4, 2023
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-mDlx-GCaMP6f-Fishell-2 (AAV1)

    Viral Prep
    #83899-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-mDlx-GCaMP6f-Fishell-2 (#83899). In addition to the viral particles, you will also receive purified pAAV-mDlx-GCaMP6f-Fishell-2 plasmid DNA. mDlx-driven expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    None
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-mDlx-GCaMP6f-Fishell-2 (AAV9)

    Viral Prep
    #83899-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-mDlx-GCaMP6f-Fishell-2 (#83899). In addition to the viral particles, you will also receive purified pAAV-mDlx-GCaMP6f-Fishell-2 plasmid DNA. mDlx-driven expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    None
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only

  41. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)

    Viral Prep
    #83897-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  42. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)

    Viral Prep
    #83896-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV-mDlx-GFP-Fishell-1 (AAV9)

    Viral Prep
    #83900-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    GFP
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only

  44. pAAV-mDlx-GFP-Fishell-1 (AAV1)

    Viral Prep
    #83900-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    GFP
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  45. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV9)

    Viral Prep
    #83898-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    mCherry
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  46. pAAV-mDlx-GFP-Fishell-1 (AAV2)

    Viral Prep
    #83900-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    GFP
    Available since
    Aug. 25, 2023
    Availability
    Academic Institutions and Nonprofits only

  47. pAAV-hDlx-Flex-dTomato-Fishell_7 (AAV9)

    Viral Prep
    #83894-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-Flex-dTomato-Fishell_7 (#83894). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-dTomato-Fishell_7 plasmid DNA. hDlx-driven, Cre-dependent expression of dTomato. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato (Cre-dependent)
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV1)

    Viral Prep
    #83898-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    mCherry
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  49. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV1)

    Viral Prep
    #83897-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV-hDlx-Flex-GFP-Fishell_6 (AAV9)

    Viral Prep
    #83895-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    GFP (Cre-dependent)
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 850 of 867 results