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Plasmid#60029PurposeCYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
UseTagsFLAGExpressionMammalianMutationCysteine 601 changed to AlaninePromoterTREAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-TMPRSS2
Plasmid#187484PurposeExpression vector - TMPRSS2DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
374 pME-ets1a
Plasmid#49000Purposefull length coding sequence for zebrafish ets1a gene in gateway middle entry vectorDepositorInsertv-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-GLI1
Plasmid#79497PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDSDepositorInsertGLI1 (GLI1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-C601A
Plasmid#60028PurposeN-terminal GFP tagged catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
UseTagsGFPExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FOS
Plasmid#79511PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDSDepositorInsertFOS (FOS Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
UseTagsExpressionWormMutationPromoterodr-3Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239
Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1
Plasmid#121831PurposepMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
Plasmid#194755PurposeLentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCav1
Plasmid#194290PurposeMultisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRTDepositorInsertcaveolin1 (cav1.S Frog)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-HA-TEV
Plasmid#105769PurposeExpression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsHA-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorInsertCCL5 Promoter (CCL5 Synthetic, Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCCL5 PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPRR2A-pFastBac I
Plasmid#182460PurposeExpression His-tagged SPRR2A in insect cellsDepositorInsertsmall proline rich protein 2A (SPRR2A Human)
UseTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L
Plasmid#121833PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/Dnmt3a-3L (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)
Plasmid#174336PurposeThe ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2DepositorInserthuman Orai1 (ORAI1 Human)
UseRetroviralTagspHluorin inserted in the extracellular loop 2 of …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only