Addgene

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Showing: 3361 - 3380 of 10598 results

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pAAV.GFAP.iGABASnFR.F102G

Plasmid

#112173

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

Expression

Mutation

F102G

Promoter

GFAP

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-p54

Plasmid

#35379

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

p54 (NONO Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

A464V

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP Δvpu/env

Plasmid

#101345

Purpose

Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta vpu/Env

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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hTPST1

Plasmid

#11252

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST1 (TPST1 Human)

Use

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-flex-Positron-ST

Plasmid

#129267

Purpose

Cre-dependant expression of soma-localized Positron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

AAV

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

hsyn

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

Plasmid

#136627

Purpose

Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-R59

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

Plasmid

#81133

Purpose

mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
VAV2 DH/PH/C1 (VAV2 Human)

Use

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLX307 LacZ

Plasmid

#117730

Purpose

Open reading frame vector encoding LacZ

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

LacZ

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1c

Plasmid

#134832

Purpose

Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Sci. 1995 Dec;108 ( Pt 12):3775-8

Insert

Myo 1c (rat) with 3' UTR (Myo1c Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLiLucB

Plasmid

#31596

Depositor

Michele Calos

Article

Woodard et al PLoS One. 2010 . 5(6):e11367.

Insert

alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Tab1-3'UTR

Plasmid

#97155

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Tab1 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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TAp63gamma-(3X)HA

Plasmid

#26977

Depositor

David Sidransky

Article

Upadhyay et al Biochem Biophys Res Commun. 2007 May 18.

Insert

TAp63gamma (TP63 Human)

Use

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98

Plasmid

#130704

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2mut

Plasmid

#31819

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

two CTATATTTAG RSRF sites mutated to CGATATTTCG

Promoter

mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWZLneo Flag mPTIP

Plasmid

#15558

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Use

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-NICD1

Plasmid

#46048

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Entry vector

Tags

Myc tag

Expression

Mutation

Cytoplasmic domain initiating at amino acid 1753

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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RNF34

Plasmid

#119937

Purpose

RING finger protein 34 expression

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only