pAAV-hsyn-flex-Positron-ST
(Plasmid
#129267)
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PurposeCre-dependant expression of soma-localized Positron in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129267 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-hsyn
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePositron-ST
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SpeciesSynthetic
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MutationD92N, E199V
- Promoter hsyn
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: NheI, 3' cloning site: HindIII.
Please visit https://www.biorxiv.org/content/10.1101/690925v1.full to read the preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hsyn-flex-Positron-ST was a gift from Eric Schreiter (Addgene plasmid # 129267 ; http://n2t.net/addgene:129267 ; RRID:Addgene_129267) -
For your References section:
A general approach to engineer positive-going eFRET voltage indicators. Abdelfattah AS, Valenti R, Zheng J, Wong A, Podgorski K, Koyama M, Kim DS, Schreiter ER. Nat Commun. 2020 Jul 10;11(1):3444. doi: 10.1038/s41467-020-17322-1. 10.1038/s41467-020-17322-1 PubMed 32651384