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PurposeCre-dependant expression of Voltron in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-hsyn
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVoltron
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SpeciesSynthetic
- Promoter hsyn
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: NheI, 3' cloning site: HindIII
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hsyn-flex-Voltron was a gift from Eric Schreiter (Addgene plasmid # 119035 ; http://n2t.net/addgene:119035 ; RRID:Addgene_119035) -
For your References section:
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging. Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens MB, Lavis LD, Schreiter ER. Science. 2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416. 10.1126/science.aav6416 PubMed 31371562