Addgene

We narrowed to 5,229 results for: hwa

Showing: 3361 - 3380 of 5229 results

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pGEX4T-Mst2 SARAH

Plasmid

#84298

Purpose

Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst2 (STK3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L443W

Promoter

tac

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
GST-CHMP4A (205-222) (pGEX2T(TEV))

Plasmid

#80633

Purpose

Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 205-222;

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG252

Plasmid

#80584

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG253

Plasmid

#80585

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP (N162D)-GFP

Plasmid

#73580

Purpose

Lentivirus expression of mOC-STAMP with N162D mutation

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mutation

changed asparagin (N) 162 to Aspartic Acid (D)

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pUAST-HA, Pbl-A DH domain

Plasmid

#69794

Purpose

Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Depositor

Arno Muller

Article

van Impel et al Development. 2009 Mar;136(5):813-22. doi

Insert

Pebble DH domain

Use

P element-based puast vector for gal4-regulated e…

Tags

HA-Tag

Expression

Insect

Mutation

Contains only the Pebble DH domain

Promoter

hsp70

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
pQCXIB-FLAG-hDIXDC1-L-WT

Plasmid

#61224

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

March 31, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shDIXDC1-Hu-16

Plasmid

#61237

Purpose

Entry clone encoding shRNA to human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-Blast-FLAG-hDIXDC1 WT

Plasmid

#61220

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

PGK

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-Hygro-FLAG-hDIXDC1 WT

Plasmid

#61213

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT

Plasmid

#61218

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV/TO

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-F630S

Plasmid

#61241

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Expression

Mutation

Changed Phenylalanine 630 to Serine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-S592A

Plasmid

#61240

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Expression

Mutation

Changed Serine 592 to Alanine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-WT

Plasmid

#61239

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Expression

Mutation

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-S592A

Plasmid

#61211

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Expression

Mutation

Changed Serine 592 to Alanine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-F630S

Plasmid

#61212

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Expression

Mutation

Changed Phenylalanine 630 to Serine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-H122Y

Plasmid

#33085

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H122Y

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEN_hU6miR-Gb2-K

Plasmid

#25759

Purpose

Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CTLA-4-mGFP

Plasmid

#223594

Purpose

Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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