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Plasmid#190720PurposeFor microglia differentiation from iPSCDepositorInsertMAFB-IRF8-CEBPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL
Plasmid#34593DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
Plasmid#84239PurposeExpresses ABA-inducible VPR-Sp dCas9DepositorInsertsABI-tagBFP-Sp dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18 LOF
Plasmid#131729PurposeExpresses a loss of function mutant of an engineered variant of RAD18 ("e18")DepositorInserte18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsp27 wt FL
Plasmid#17444DepositorInsertsmall heat shock protein27 (HSPB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR(T790M)
Plasmid#32070DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationT790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TDP-43
Plasmid#190093PurposeExpresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.DepositorInsertTAR DNA-Binding Protein 43 (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc
Plasmid#21850DepositorInsertATF4 (Atf4 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationATF4 5' UTR and AUG fused to Luciferase driv…PromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJZC74
Plasmid#62342PurposesgRNA + 1x COM with COM-KRAB effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-KRAB
UseLentiviralTagsKRABExpressionMammalianMutationTargets sv40 promoter, sequence: GAATAGCTCAGAGGCC…PromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD47-bio-His
Plasmid#53150PurposeExpresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD47 (CD47 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC116
Plasmid#62344PurposesgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFPDepositorInsertssgRNA + 2x MS2 (wt+f6) binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGCPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-HA-Smad2
Plasmid#11734DepositorInsertSMAD2 (SMAD2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-EGFP-eDHFR(69K6)-MCS
Plasmid#172100PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)-20aa
UseTagsExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
UseTagsExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PTPRC-bio-His
Plasmid#51813PurposeExpresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTPRC (PTPRC Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_SNCA:EGFP
Plasmid#92195PurposeLentiviral overexpression vector to make stable bicistronic cell line for α-Syn screenDepositorInsertDsRed-IRES-SNCA-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP-NLS-mut
Plasmid#120832PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5-eYFP "NLS-mut"
UseTagseYFP-V5ExpressionMammalianMutationR372A/Q373A/K387A/H388A/K389APromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
TMED5-bio-His
Plasmid#53338PurposeExpresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED5 (TMED5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only