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Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags6xhisExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1
Plasmid#87737PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO- flag-hDDI2-D252N_pET11a
Plasmid#169123Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationD252NPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1b2 (273-586)
Plasmid#162733PurposeBacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1b2 domains (residues 273-586)
UseTags6HisExpressionBacterialMutationCodon optimisedPromoterAvailable sinceMarch 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
UseTagsExpressionBacterialMutationStop codon to glutamine at 23rd amino acidPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-ybbr-HIS-ddFLN4-K10
Plasmid#101718PurposeK10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorInsertHOXC9 (Hoxc9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorInserthuman mature (delta19) ETFalpha (ETFA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2Z)
Plasmid#29776DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-1066 (full length)
Plasmid#46171DepositorInsertVcl (VCL Chicken)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1-plus
Plasmid#126775PurposeExpression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.DepositorInsertSpCas9-HF1-plus
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A; Q695A; Q926A; amino acids 1005-1013 replac…PromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
UseTagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-ddFLN4-HIS-ybbr
Plasmid#101239Purposewildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dHer1cys
Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
UseTags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPV00605 pET21GG2-His(6)-BECN(1-265)
Plasmid#136686PurposeExpresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 IDR + CC (BECN1 Human)
UseTagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PET-B-SpCas9-HF1-NLS-6xHis
Plasmid#207385PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-SpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926A, amino acids 1005-1013…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11-His6-Pif6-sfGFP-ssrA
Plasmid#140891PurposeExpression of PIF-sfGFP-ssrA in E. coliDepositorInsertPIF-sfGFP-ssrA
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only