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Plasmid#119763PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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pGEX6P1-DEST-FLAG
Plasmid#119754PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG545
Plasmid#40116DepositorTypeEmpty backboneUseGateway donor vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionMutationPromoterAvailable sinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pStart-K
Plasmid#20346DepositorTypeEmpty backboneUseMouse Targeting; Gateway cloningTagsExpressionMutationPromoterAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Myc
Plasmid#119752PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
372 pTol2-fli1ep:EGFPDest
Plasmid#73491PurposeTol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signalDepositorInsertfli1ep:EGFP-attR1-attR2
UseTagsExpressionMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-HA
Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGW_SM_BamHI
Plasmid#49315PurposeSubcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insertDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN41A-T10M
Plasmid#27365DepositorInserttetR(sB)
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P1
Plasmid#27366DepositorInsertPmyc1tetO
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONRHYG
Plasmid#29636DepositorTypeEmpty backboneUseGateway donorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pBSWt1-Dest
Plasmid#59764PurposePCR template for amplifying the attL1-em7-kanr-attL2-pA cassetteDepositorInsertattL1-em7-kanR-attL2-pA
UseTagsExpressionMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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252 pTol2-MTDest
Plasmid#73485Purposepromoterless Tol2 vector with 6xmyc tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsert6xmyc/attR1/attR2
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p49202-DEST
Plasmid#90100PurposeGateway destination vector featuring 49202 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.DepositorTypeEmpty backboneUseGateway destination vector for protein expression…TagsExpressionMutationPromoter49202Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGDestSC-ATG
Plasmid#49322PurposeDestination vector for Golden Gate assembly; Few restriction sites for subcloning.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-NAT1-C-HA
Plasmid#195178PurposeVector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorInsertNAT1 (NAT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-jAspSnFR3
Plasmid#203460PurposejAspSnFR3 in Gateway cloning entry vectorDepositorInsertjAspSnFR3
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS
Plasmid#132927PurposepMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInserthumanized SpCas9
UseCRISPR and Synthetic Biology; Pmagic gateway entr…TagsExpressionMutationPromoterAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only