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Plasmid#71581PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges serine-71 to alaninePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK517
Plasmid#71579PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges glutamate-435 to aspartatePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK514
Plasmid#71577PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges glutamine-435 to alaninePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK512
Plasmid#71575PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges arginine-228 to glutamatePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H09
Plasmid#71003PurposeGateway entry cloneDepositorInsertSNF4Agamma (SNF4Agamma Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C07
Plasmid#70985PurposeGateway entry cloneDepositorInsert14-3-3zeta (14-3-3zeta Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2B09
Plasmid#70918PurposeGateway entry cloneDepositorInsertgro (gro Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C03
Plasmid#70959PurposeGateway entry cloneDepositorInsertTaf4 (Taf4 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5B
Plasmid#67326PurposehArl5b with stop codon in Gateway Entry vectorDepositorInsertARL5B (ARL5B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SFFV tdTomato L5-L2
Plasmid#62155PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloningDepositorInserttdTomato
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP L3-L2
Plasmid#62159PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 PuroR L5-L2
Plasmid#62165PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloningDepositorInsertPuromycin
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L1-L4
Plasmid#62136PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA R4-R3
Plasmid#62137PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L5-L2
Plasmid#62139PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 eGFP L3-L2
Plasmid#62145PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloningDepositorInserteGFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK-miR-30 R4-R3
Plasmid#62121PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK-miR-30 L1-R5
Plasmid#62118PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP R4-R3
Plasmid#62158PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only