-
Plasmid#56158PurposeLocalization: Endoplasmic Reticulum, Excitation: 604, Emission: 659DepositorInsertCytERM
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCNL-N1_zyxin
Plasmid#65682PurposeExpresses Zyxin-CNL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p28atDarpp
Plasmid#108318PurposeExpresses tDarpp in E.coliDepositorInserttDarpp (PPP1R1B Human)
UseTags6 HistadineExpressionBacterialMutationPromoterT7Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
spa-GCaMP6f
Plasmid#67558Purposephotoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseTagsHIS-tagExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterCMVAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6f
Plasmid#67561Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertsspa-GCaMP6f
UseTagsExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164042PurposeCRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA geneDepositorInsertmCherry-CRISPR-TagV2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
M48 GST-TEV-Chibby-N
Plasmid#17196DepositorInsertChibby (CBY1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-63PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
ShuttleMCP
Plasmid#50959PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoteruses portion of the mouse cofilin gene as a mouse…Available sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTrichoGate-13
Plasmid#160713PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X Hemagglutinin tag (HA)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Flag Smad3 EPSM
Plasmid#27026DepositorInsertSmad3 (SMAD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationT179V S204A S208A S213APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3xFlag-S242R-MEFV
Plasmid#167020PurposeGateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagExpressionMutationserine 242 in arginine (S242R)PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
M49 GST-TEV-Chibby-C
Plasmid#17197DepositorInsertChibby (CBY1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 64-126PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
ERroGFP_iE_C204Q_pCDNA3
Plasmid#48629PurposeMammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)DepositorInsertERroGFP
UseTagsFLAGExpressionMammalianMutationinsertion E147B, C204QPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NFLAG TRF1 deltaA deltaM
Plasmid#16065DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMutationDeleted acidic domain (aa1-66) and myb domain (aa…PromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_Mito
Plasmid#65670PurposeExpresses Mito-CNL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
ERroGFP_C147S_pCDNA3
Plasmid#48630PurposeMammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)DepositorInsertERroGFP
UseTagsFLAGExpressionMammalianMutationC147SPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)
Plasmid#111255PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptideDepositorInsertTorT signal peptide
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Tubulin-6
Plasmid#55512PurposeLocalization: Microtubules, Excitation: 462, Emission: 492DepositorInsertTubulin (TUBA1B Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
ERrefGFP1_pCDNA3
Plasmid#48635PurposeMam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFPDepositorInsertERrefGFP1
UseTagsFLAGExpressionMammalianMutationC147S,C204QPromoterAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only