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Plasmid#200508PurposeLentiviral vector expressing gRNA targeting human ARID1A and ARID1BDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET32bplus_trx_dAChE4
Plasmid#83917PurposeEncodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coliDepositorInsertDesigned hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
UseTagsHis, S-tag, and TRXExpressionBacterialMutationV13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…PromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-D1-IRES-SS-D2-IRES-HA-D3
Plasmid#215119PurposeExpresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.DepositorUseLentiviralTagsFLAG-tag, HA-tag, and SS (STREP-STREP)ExpressionMammalianMutationPromoterIRES and TREG3Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS1153
Plasmid#29068PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle36 (CRH Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1156
Plasmid#29071PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle39 (CRH Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1154
Plasmid#29069PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle37 (CRH Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_LEO1:1-666
Plasmid#211074PurposeMammalian expression of full-length human LEO1. C-terminal HaloTag.DepositorInsertLEO1:M1-D666 (LEO1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTN-HaloTag_LEO1:1-666
Plasmid#211073PurposeMammalian expression of full-length human LEO1. N-terminal HaloTag.DepositorInsertLEO1:M1-D666 (LEO1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DENND3:894-1198
Plasmid#211203PurposeMammalian expression of human DENND3 fragment V894 to E1198. N-terminal HiBiT tag.DepositorInsertDENND3:V894-E1198 (DENND3 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DENND3:894-1198
Plasmid#211277PurposeMammalian expression of human DENND3 fragment V894 to E1198. C-terminal HiBiT tag.DepositorInsertDENND3:V894-E1198 (DENND3 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS1155
Plasmid#29070PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle38 (CRH Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 F276C 3xFlag
Plasmid#110064PurposeFGFR2 F276C expression in mammalian cells (JCOPO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Phenylalanine 276 to Cysteine (F276C)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K41E 3xFlag
Plasmid#110105Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 41 to Glutamic acid (K41E)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8
Plasmid#111903PurposeFull length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.DepositorInsertsUseTags8xHis, SNAPf-tag, TEV site, and ZZ-tagExpressionInsectMutationOptimised for expression in Sf9 cells.PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_hsHAUScomplex_FL
Plasmid#202203PurposeBaculoviral transfer vector to co-express 8 subunits of human augmin complexDepositorInsertsHAUS1 (HAUS1 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS3 (HAUS3 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS2 (HAUS2 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS6 (HAUS6 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS7 (HAUS7 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS4 (HAUS4 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS5 (HAUS5 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS8 (HAUS8 Human, codon-optimized form for expression in Trichoplusia ni cells)
UseTags-mGFP-TEV-TwinStrep and His-tag (6x)ExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNIL
Plasmid#172113PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expressionUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dynein-2_complex
Plasmid#132536PurposeFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.DepositorInsertsUseTags8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only