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Plasmid#70226PurposeExpresses C-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
UseTagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Ile 129 to Cys, Deleted amino acids 1-128PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 SWS cone opsin
Plasmid#109363PurposeHuman SWS cone opsin with 1D4 tagDepositorInsertSWS cone opsin (OPN1SW Human)
UseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS2410-Tier1-PhCMV-Myr_mTagBFP2
Plasmid#169535PurposeTier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).DepositorInsertPCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 SV8
Plasmid#184011PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).DepositorInsertProm1 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationWild type cDNAPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH72-Tier1-PhCMV-SEAP
Plasmid#169560PurposeTier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).DepositorInsertPCMV-driven human secreted placental alkaline phosphatase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
mH2A2-CT-MYC
Plasmid#45170DepositorUseTagsHIS and MYCExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PH-dL5**
Plasmid#96947PurposeExpresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsEGFP and dL5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-optoB2-LYY-YFP
Plasmid#75271PurposeG-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224ADepositorInsertG-protein independent version of optoB2
UseTagseYFPExpressionMammalianMutationpoint mutations at L72F,Y136G,Y224APromoterAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR2
Plasmid#59012PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR2 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorInsertGFAP (Gfap Mouse)
UseTagsHaloTagExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 L232A
Plasmid#8976DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationL232APromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
PH-dL5**
Plasmid#96946PurposePLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsdL5 and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH2.20_mCherry-MS2x4
Plasmid#205535PurposeExpress mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP2-NbV5
Plasmid#201478PurposeBRET2 detection of V5-tagged proteinDepositorInsertGFP2-NbV5
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rluc8-NbV5
Plasmid#201479PurposeBRET detection of V5-tagged proteinDepositorInsertNbV5-Rluc8
UseTagsRluc8ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Septin 11 isoform III
Plasmid#120319PurposeSeptin 11 isoform III mammalian expressionDepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FRB-VMAc-CSarcin
Plasmid#70223PurposeExpresses VMA intein with C-terminal portion of alpha-sarcinDepositorInsertalpha Sarcin (C-terminal portion)
UseTagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Asp 28 to Cys, deleted amino acids 1-27PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Vh-HA-2xFRB
Plasmid#20224DepositorInsert2xFRB (MTOR Human)
UseTagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only