Addgene

We narrowed to 14,719 results for: LEA

Showing: 3241 - 3260 of 14719 results

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pJH26_pBad_AraC_Para-sfGFP.1TAG

Plasmid

#163721

Purpose

Arabinose-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.1TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH27_pBad_AraC_Para-sfGFP.2TAG

Plasmid

#163722

Purpose

Arabinose-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.2TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG,Y151TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH25_pBad_AraC_Para-sfGFP.0TAG

Plasmid

#163720

Purpose

Arabinose-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II DC

Plasmid

#35181

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal domain repeats 1-22 and 31-52

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Use

Tags

6xHis-TEV

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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IZ1181

Plasmid

#21049

Depositor

Tadahide Izumi

Article

Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

Insert

human MDM2 (MDM2 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

no mutation. This is the wild-type human MDM2.

Promoter

Available since

July 9, 2009

Availability

Academic Institutions and Nonprofits only
pCas9–mKate2ps–EgRNA

Plasmid

#62716

Purpose

Empty; no U6 gRNA cassette

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pIS0 - HOXA7 3'UTR

Plasmid

#12079

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Insert

HOXA7 3'UTR (HOXA7 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pIS0 - HOXB8 3'UTR

Plasmid

#12076

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Insert

HOXB8 3'UTR (HOXB8 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pTORKm43GW

Plasmid

#112897

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTC208

Plasmid

#70017

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTAL3

Plasmid

#31034

Depositor

Daniel Voytas, Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALEN-FokI nuclease backbone

Use

Tags

AcV5 and FokI nuclease

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pFH42

Plasmid

#125881

Purpose

Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-MseLig1

Plasmid

#107071

Purpose

Expresses M. sedula DNA Ligase 1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

DNA ligase 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-Cb5-pEGFP-C1

Plasmid

#166764

Purpose

To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

Depositor

David Andrews

Insert

Cb5 tail anchor (Cyb5a Rat)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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