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Plasmid#169043Purpose5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.DepositorInsertMnx1 enhancer (3x) (mnx1 Zebrafish)
UseGateway entry cloning vectorTagsExpressionMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3
Plasmid#127354Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP Halo
Plasmid#104098PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.DepositorInsertstdMCP-Halo
UseLentiviralTagsC-terminal Halo-tag, N-terminal NLS, and Two syno…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3B
Plasmid#137757PurposeExpression of LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-GABARAPL1
Plasmid#137759PurposeExpression of GABARAPL1DepositorInsertGABARAPL1 (GABARAPL1 Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdPCP stdGFP
Plasmid#104099PurposeLentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.DepositorInsertstdPCP-stdGFP
UseLentiviralTagsN-terminal NLS, Two synonymous copies of GFP (C-t…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53SS(1-393)
Plasmid#200311PurposeBacterial expression of human superstable p53 as His6GB1tev fusionDepositorInsertp53 (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3A
Plasmid#137756PurposeExpression of LC3ADepositorInsertMAP1LC3A (MAP1LC3A Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-tagRFP-KDEL
Plasmid#114177PurposeGateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseGateway entry cloneTagsKDEL retention signal and signal peptideExpressionMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP1.GFP
Plasmid#109139PurposeGFP-tagged CYFIP1 expression constructDepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3
Plasmid#127356Purposegateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262-ABE8e
Plasmid#161523PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editingDepositorInsertABE8e-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e-zspcas9(d10a) en…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-GABARAPL2
Plasmid#137761PurposeExpression of GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3
Plasmid#127353Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and mVenusExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E ubb polyA
Plasmid#195977PurposeGateway p3E vectorDepositorInsertubb:polyA (ubb Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNoneExpressionMutationPromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD
Plasmid#159999PurposeExpresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertSpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationK842M mutation (Catalytic Inactive)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNB1
Plasmid#192301PurposeGateway entry vector for an inducible 3xFLAG-KPNB1DepositorInsertKPNB1 (KPNB1 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only