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Plasmid#162979PurposeGateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoterDepositorInsertTet-regulated TRE3G minimal promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
UseTagsExpressionYeastMutationValine 163 changed to alaninePromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Paxillin
Plasmid#186143PurposeFor expresseion of recombinant paxillin in bacteriaDepositorInsertPXN (PXN Human)
UseTagsGSTExpressionBacterialMutationPaxillin C1997A (silent mutation for cloning)PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH86
Plasmid#128199Purposeimproved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)DepositorInsertimproved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS23·Pm-GFP
Plasmid#122593PurposeExpresses GFP under the regulatory control of Pm. Expression needs additional xyls geneDepositorInsertPm promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse NHERF-1
Plasmid#32950DepositorInsertSlc9a3R1 (Nherf1 Mouse)
UseTagsnoneExpressionBacterialMutationnonePromotert3Available sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERas-IH
Plasmid#15688DepositorInsertERas (Eras Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcryBFP_Umyo18bGFP
Plasmid#172527PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.DepositorInsertmyo18b
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-STIL
Plasmid#80265Purposebaculovirus creation plasmid for GST-STILDepositorInsertSTIL (STIL Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPKAnes
Plasmid#61092PurposeGFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signalDepositorInsertpkac2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH88
Plasmid#128201PurposesgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F3 LUC
Plasmid#22429DepositorInsertF3 (LOC110973015 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceMay 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
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C413-E31: attB4-TRE3Gp>-attB1r
Plasmid#162924PurposeGateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoterDepositorInsertTet-regulated TRE3G minimal promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
S12_EcoRV_PGK_AscI
Plasmid#128476PurposePGK promoterDepositorInsertPGK promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PmarA-cfp
Plasmid#79867PurposeDegradation tagged CFP fluorescent reporter for MarADepositorInsertA modified marRAB promoter upstream of a degradation tagged cfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermodified marRAB promoter with inactivated MarR bi…Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only