Addgene

We narrowed to 4,926 results for: pcDNA3.1+

Showing: 3221 - 3240 of 4926 results

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mH2A1.2-CT-MYC

Plasmid

#45168

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Use

Tags

HIS and MYC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-LOV-VP16

Plasmid

#42499

Purpose

LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

FKF1 (N-LOV, G128D mutant) fused with VP16

Use

Tags

F-type VP16 activation domain and HA tag

Expression

Mammalian

Mutation

G128D 1-168aa

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-Tetra-com-vector

Plasmid

#132553

Purpose

Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

Cas9
sgRNA scaffold with com replacing the Tetraloop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2396-Tier1-PhCMV-4xCOX8_Ypet

Plasmid

#169534

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized yellow fluorescent protein Ypet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 D319N

Plasmid

#8979

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

D319N

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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hGFAP R239C

Plasmid

#169476

Purpose

Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 239 to C

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA flag-Pcbp2

Plasmid

#184028

Purpose

Codon optimized Pcbp2 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Pcbp2 (Pcbp2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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Galphai2-mTurquoise2

Plasmid

#69621

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai2-mTurquoise2

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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perbB2K732M-ECFP

Plasmid

#40269

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Use

Tags

ecfp

Expression

Mammalian

Mutation

K732M

Promoter

CMV

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-3xflagGREB1a

Plasmid

#112221

Purpose

mammalian expression of flag tagged GREB1a

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1a (GREB1 Human)

Use

Tags

3x flag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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HA-EcPPK1

Plasmid

#159279

Purpose

Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

Depositor

Michael Downey

Article

Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

Insert

HA-tagged Polyphosphate Kinase 1

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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DAXX-myc-his

Plasmid

#1852

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
mc2 blast EF1a

Plasmid

#206223

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP670nano3

Plasmid

#184668

Purpose

LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-LC-Myosin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK

Plasmid

#62670

Purpose

Vector for PhiC31o mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

PhiC31

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos DD

Plasmid

#8968

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HCMVgH (Merlin)

Plasmid

#136448

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Use

Tags

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only