-
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry
Plasmid#50458PurposeDouble floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas ServiceAAV8InsertrM3D-mCherry
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-FilaminA-N-9
Plasmid#54098PurposeLocalization: Actin/Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertFilaminA (FLNA Human)
UseTagsmEmeraldExpressionMammalianMutationD2626HPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterrrnB P1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionBacterialMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV1)
Viral Prep#100848-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3-myr-AKT-HA
Plasmid#31789DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTHD422N
Plasmid#194432PurposeAPOBEC1 fused to YTHD422NDepositorInsertAPOBEC1-YTHD422N (APOBEC1 Human)
UseCRISPRTagsAPOBEC1-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…PromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
Mm-PylRS-AF/Pyl-tRNACUA
Plasmid#122650PurposetRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.DepositorInsertPylRS-AF, Pyl-tRNACUA
UseTagsFLAGExpressionMammalianMutationTyrosine 306 changed to Alanine, Tyrosine 384 cha…PromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Cav3.2 Ca2+ channel [N55/10R]
Plasmid#177562PurposeMammalian Expression Plasmid of anti-Cav3.2 Ca2+ channel (Human). Derived from hybridoma N55/10.DepositorInsertanti-Cav3.2 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNA1H Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn GAPDH
Plasmid#220918PurposeNeuronal specific expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmartBAC-S
Plasmid#107268PurposeBroad host range bacterial artificial chromosome vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV5)
Viral Prep#114469-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mPiezo1-IRES-eGFP
Plasmid#80925Purposeexpresses mouse Piezo1 with GFPDepositorInsertmouse Piezo1 CDS (Piezo1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bJunVN173
Plasmid#22012DepositorInsertbJunVN173 (Jun Aequorea Victoria, Rat)
UseTagsFLAGExpressionMammalianMutationc-Jun(257-338)-Venus(1-172)PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
UseTagsExpressionBacterialMutationH840A of Cas9PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2161
Plasmid#141950PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL1 (GRHL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_membrane_EGFP
Plasmid#139402Purposein vitro transcription for mRNADepositorInsertmembrane-EGFP
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterT3Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorInsertZNF598 (ZNF598 Human)
UseTagsTEV-3xFLAGExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only