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pCE-hSK

Plasmid

#41814

Purpose

Non-integrating (episomal) expression of human SOX2 and KLF4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

SOX2, KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCE-hSK

Plasmid

#41814

Purpose

Non-integrating (episomal) expression of human SOX2 and KLF4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

SOX2, KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Insert

nucA

Use

Tags

His and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized ecoli residues - 22-266

Promoter

T7

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Chk1-Flag

Plasmid

#22894

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Chk1 (CHEK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Chk1-Flag

Plasmid

#22894

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Chk1 (CHEK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II WT

Plasmid

#35175

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II WT

Plasmid

#35175

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-D4H*-mCherry

Plasmid

#134604

Purpose

fluorescent cholesterol-binding probe

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

D4H*

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

Y415A and A463W

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-D4H*-mCherry

Plasmid

#134604

Purpose

fluorescent cholesterol-binding probe

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

D4H*

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

Y415A and A463W

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, David Root, William Hahn

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, David Root, William Hahn

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

Viral Prep

#26971-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

CaMKII

Tags

EYFP

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only
pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge