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Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD127
Plasmid#69175PurposeIntegration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg27-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK148-Pnmt41-GBP-mCherry(N)-leu1+
Plasmid#89070Purposegenomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
LD111
Plasmid#69165PurposeIntegration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg11-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-HBH
Plasmid#26886DepositorInsertHBH tag N-terminal
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAV0543
Plasmid#133493PurposeExpression of nucleo-cytoplasmic mCherry in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0761
Plasmid#133494PurposeExpression of nucleo-cytoplasmic mTagBFP2 in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0479
Plasmid#133499PurposeExpression of sfGFP excluded from the nucleus with two NES signalsDepositorInsertpHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD220
Plasmid#69188PurposeIntegration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg20-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*
Plasmid#89071Purposegenomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUPD-ADH2t (17-21)
Plasmid#101701PurposeADH2t in domestication vectorDepositorInsertGCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-PGK1 (01-11)
Plasmid#101689PurposepPGK1 in domestication vectorDepositorInsertGGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-CU2-Pleum
Plasmid#60328PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promoteā¦TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV0756
Plasmid#133508PurposeExpression of 3mCherry tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
DdMCU
Plasmid#67642PurposeDdMCU-Flag in pACT2 vector human codon optimisedDepositorInsertDdMCU
UseTagsFlag tag with linkerExpressionYeastMutationPromoterADH1Available sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only