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pDONR223_HAPLN1_p.S326N

Plasmid

#81640

Purpose

Gateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S326N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAPLN1_p.K115N

Plasmid

#81646

Purpose

Gateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K115N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAPLN1_p.P233H

Plasmid

#81648

Purpose

Gateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P233H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARSI_p.P228L

Plasmid

#81569

Purpose

Gateway Donor vector containing ARSI ,part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARSI (ARSI Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P228L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARSI_p.V373M

Plasmid

#81481

Purpose

Gateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARSI (ARSI Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V373M

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CASK gRNA (BRDN0001149157)

Plasmid

#77401

Purpose

3rd generation lentiviral gRNA plasmid targeting human CASK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CASK (CASK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CASK gRNA (BRDN0001145000)

Plasmid

#77402

Purpose

3rd generation lentiviral gRNA plasmid targeting human CASK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CASK (CASK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CASK gRNA (BRDN0001148068)

Plasmid

#77403

Purpose

3rd generation lentiviral gRNA plasmid targeting human CASK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CASK (CASK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 4A

Plasmid

#59907

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated four serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-Lam

Plasmid

#133902

Purpose

Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-WT

Plasmid

#90331

Purpose

expresses Wild type BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Aequorea victoria, Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Lamin-A

Plasmid

#206027

Purpose

Expresses GFP-tagged Lamin A in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Lamin-A (LMNA Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUCM-CLYBL-hNIL

Plasmid

#105841

Purpose

Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

CAG, EF-1alpha, and TRE3G

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHuji

Plasmid

#130902

Purpose

Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHuji (CD63 Human, synthetic)

Use

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Gln-36 and Leu-37 in extra…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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tetO-ASCL1-LMX1b-NURR1-PuroR

Plasmid

#182298

Purpose

Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

Depositor

Kristen Brennand

Article

Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis

Plasmid

#157024

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

JAG1 (JAG1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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