Addgene

We narrowed to 4,926 results for: pcDNA3.1+

Showing: 3181 - 3200 of 4926 results

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VinTS-tCRMod-GGSGGS9

Plasmid

#111765

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.214_mCherry-PP7x12

Plasmid

#205556

Purpose

Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Use

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Promoter

Available since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LgBit-NbV5

Plasmid

#201476

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbV5

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA Set9 mutant

Plasmid

#24085

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H297A

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-EGFP-RIP2

Plasmid

#131201

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 (RIPK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon

Plasmid

#206218

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 T3550A/S3556A

Plasmid

#199574

Purpose

Expresses FLAG-tagged mutated SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Opto-casp9_VfAU1-LOV_casp9

Plasmid

#131220

Purpose

Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (VfAU1-LOV_casp9)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.19_mCherry-MS2x2

Plasmid

#205534

Purpose

Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-TIAL1

Plasmid

#207669

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

TIAL1 (TIAL1 Human)

Use

Tags

MCP-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6B

Plasmid

#42022

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6B (TNRC6B Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon hPGK

Plasmid

#206230

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas8

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-CytERM-mCherry-(F30-2xPepper)10

Plasmid

#129403

Purpose

CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

CytERM-mCherry-(F30-2xPepper)10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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mH2A1.1-CT-MYC

Plasmid

#45166

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Use

Tags

HIS and MYC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-MRGBP

Plasmid

#15325

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

Insert

MRG-binding protein (MRGBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pSL2622 (pCas7_Vch)

Plasmid

#200894

Purpose

Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas7

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-ICUE

Plasmid

#118480

Purpose

ddRFP-based single-color biosensor for monitoring cAMP dynamics.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-ICUE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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