Addgene

We narrowed to 6,150 results for: mag

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psiCHECK2-miR-34 MT

Plasmid

#78259

Purpose

psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

mutated miR-34 target site

Use

Luciferase; Microrna activity

Tags

Expression

Mammalian

Mutation

nucleotides 2-5 and 10-11 changed from CCGT to GG…

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

Plasmid

#128609

Purpose

Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

uORF1-36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 LC EPSM

Plasmid

#27038

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; T179V S204A S208A S213A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pBABE puro Brca1 S1423A S1524A HA

Plasmid

#41968

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

S1423A, S1524A

Promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL EPSM

Plasmid

#27006

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; T179V S204A S208A S213A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

Plasmid

#164041

Purpose

CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

mCherry-CRISPR-TagV2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pPAP_BGL1

Plasmid

#183735

Purpose

Expresses Aspergillus aculeatus β-glucosidase (BGL1)

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

β-glucosidase (AaBGL1)

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1 promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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mTFP1-H3-23

Plasmid

#55489

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H3 (H3C2 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pBacT7-S1T1L

Plasmid

#33292

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pC1-FGB (FlincG2)

Plasmid

#49204

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG2 (PRKG1 Bovine, GFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_251

Plasmid

#180546

Purpose

Entry vector containing mScarlet

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pzyxin-emiRFP703

Plasmid

#136568

Purpose

Zyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

zyxin-emiRFP703

Use

Tags

emiRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-ER-14

Plasmid

#56432

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

ER (CALR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hSynapsin1-axon-GCaMP6m

Plasmid

#111261

Purpose

For enriched expression of GCaMP6m in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6m

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

Plasmid

#124493

Purpose

Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipYFP(TEV cleavage seq) T2A mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_PP7-mCherry

Plasmid

#107605

Purpose

Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

PP7-mCherry

Use

Synthetic Biology

Tags

mCherry

Expression

Mutation

PP7: [delta]67_74 (non-aggregating)

Promoter

hUBC

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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