Addgene

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CASK scFv [K56A/50]

Plasmid

#190491

Purpose

Mammalian Expression of CASK scFV. Derived from hybridoma K56A/50.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

CASK (Homo sapiens) recombinant scFV (CASK Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/57R]

Plasmid

#188217

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/57

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/57]

Plasmid

#190283

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/57.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant Mouse monoclonal antibody (CASK Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.337T>C-Flag

Plasmid

#169169

Purpose

Expresses EPHX1 c.337T>C in mammalian cells

Depositor

Jeremie Gautheron, Bruno Feve

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.416A>G-Flag

Plasmid

#169170

Purpose

Expresses EPHX1 c.416A>G in mammalian cells

Depositor

Jeremie Gautheron, Bruno Feve

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.997A>C-Flag

Plasmid

#169171

Purpose

Expresses EPHX1 c.997A>C in mammalian cells

Depositor

Jeremie Gautheron, Bruno Feve

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

c.997A>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.1212G>C-Flag

Plasmid

#169172

Purpose

Expresses EPHX1 c.1212G>C in mammalian cells

Depositor

Jeremie Gautheron, Bruno Feve

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

c.1212G>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.1288G>C-Flag

Plasmid

#169173

Purpose

Expresses EPHX1 c.1288G>C in mammalian cells

Depositor

Jeremie Gautheron, Bruno Feve

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

c.1288G>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-457 tNGFR-P2A-STAT1

Plasmid

#186059

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT1 (STAT1 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-DS-HDAC5_HDAC3)

Plasmid

#114399

Purpose

For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro

Plasmid

#172624

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro281Leu

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

Plasmid

#171099

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Ascl1 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Expression

Mutation

Promoter

kdrl

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

Plasmid

#156402

Purpose

Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Gateway cloning

Tags

EYFP

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRcCMV-STAT31F

Plasmid

#134725

Purpose

Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707

Depositor

Curt Horvath, Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

STAT3-STAT1 Fusion (STAT3, STAT1 Human)

Use

Tags

FLAG and human STAT3 aa 1-514 joined to human STA…

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRcCMV-STAT13F

Plasmid

#134723

Purpose

Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707

Depositor

Curt Horvath, Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

STAT1-STAT3 fusion (STAT3, STAT1 Human)

Use

Tags

FLAG and human STAT1 aa1-508 joined to human STAT…

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) TSPAN9

Plasmid

#107502

Purpose

Expresses TSPAN9, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

TSPAN9 (TSPAN9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARSI_WT

Plasmid

#81898

Purpose

Gateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARSI (ARSI Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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