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Showing: 301 - 320 of 422 results

  4. pAGD248

    Plasmid
    #219498
    Purpose
    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219498-plasmid-map-sequence-id-435231

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  10. pDT2

    Plasmid
    #199119
    Purpose
    circular transcription template
    Depositor
    Jeff Gelles
    Article
    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12
    Insert
    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199119-plasmid-map-sequence-id-388192

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  14. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  15. pBECKP-km

    Plasmid
    #117235
    Purpose
    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
    Depositor
    Quanjiang Ji
    Article
    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii
    Insert
    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    117235-plasmid-map-sequence-id-227412

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Showing: 301 - 320 of 422 results