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Showing: 301 - 320 of 23580 results

  1. Linked VLP NES (M)

    Plasmid
    #182434
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182434-plasmid-map-sequence-id-360117

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  2. Coexpressed VLP NES (M)

    Plasmid
    #182435
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182435-plasmid-map-sequence-id-360119

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  3. Linked Free NES (M)

    Plasmid
    #182436
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    FPPS(M)-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182436-plasmid-map-sequence-id-360120

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  4. Coexpressed Free NES (M)

    Plasmid
    #182437
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    FPPS(M)
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182437-plasmid-map-sequence-id-360121

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  5. Linked VLP NES (G)

    Plasmid
    #182476
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182476-plasmid-map-sequence-id-360123

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  6. Coexpressed VLP NES (G)

    Plasmid
    #182477
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182477-plasmid-map-sequence-id-360429

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  7. Coexpressed Free NES

    Plasmid
    #182482
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182482-plasmid-map-sequence-id-360445

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  12. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. DsRed-CPI-17-wt-NES

    Plasmid
    #100787
    Purpose
    Mammalian expression of Fluorescent CPI-17 cytosolic
    Depositor
    Joseph Gordon
    Insert
    CPI-17-NES (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100787-plasmid-map-sequence-id-193216

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  14. HA-CPI-17-T38A-NES

    Plasmid
    #100779
    Purpose
    Mammalian expression of CPI-17 neutral mutant cytosolic
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38A-NES (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100779-plasmid-map-sequence-id-193200

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  15. HA-CPI-17-T38E-NES

    Plasmid
    #100777
    Purpose
    Mammalian expression of CPI-17 phospho-mimetic cytosolic
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NES (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100777-plasmid-map-sequence-id-193197

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  16. mGeos2-VEL-Farnesyl-5

    Plasmid
    #57548
    Purpose
    Localization: Membrane, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mGeos2-VEL
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57548-plasmid-map-sequence-id-218618

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  17. nes714Δ1388-1507tk/lacZ

    Plasmid
    #47616
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    714 bp from 3' end of 2nd intron with a dele…
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16

    Plasmid
    #210506
    Purpose
    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Insert
    PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210506-plasmid-map-sequence-id-415563

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Showing: 301 - 320 of 23580 results