Addgene

We narrowed to 1,464 results for: lacZ

Showing: 301 - 320 of 1464 results

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

Plasmid

#186414

Purpose

AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ E. coli)

Use

Gateway destination vector

Tags

Nuclear localization signal

Expression

Mutation

Promoter

Basal promoter of the FOG gene

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

Plasmid

#105196

Purpose

targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

Depositor

Peter Mombaerts

Article

Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

Insert

Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only
pAAVf-EnhCB-lacZnls mir-122 1xBS

Plasmid

#35643

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

1 miR-122 target site

Use

AAV

Tags

Expression

Mutation

Promoter

CB

Available since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

Plasmid

#110560

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

stRBS-lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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rI7->M71-IRES-GFP-IRES-taulacZ LNL TV

Plasmid

#15620

Depositor

Peter Mombaerts

Article

Bozza et al J Neurosci. 2002 Apr 15. 22(8):3033-43.

Insert

rI7->M71-IRES-GFP-IRES-tauLacZ LNL TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60228

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

Plasmid

#216321

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice acceptor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

Plasmid

#61441

Purpose

Dox-inducible MyoD with mRNA tag for smFISH

Depositor

Charles Gersbach

Article

Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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mGluR6 (200bp crit reg+SV40 bas prom)-LacZ

Plasmid

#18806

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

mGluR6 (200bp crit reg+SV40 bas prom)-LacZ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

Plasmid

#190790

Purpose

[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Inserts

Cas9
mazF-LVA

Use

CRISPR and Synthetic Biology

Tags

LVA

Expression

Bacterial

Mutation

silent mutations of BpiI-sites in original Cas9 g…

Promoter

PnrsB and Ptrc-[E*](104)

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

Plasmid

#60225

Purpose

Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Renilla luciferase
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

Tags

Cre-HA and Rluc-P2A

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170814

Purpose

Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mNeonGreen-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170813

Purpose

Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

Plasmid

#175175

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

sgRNA-lacZ

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP140

Plasmid

#21672

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP120

Plasmid

#21673

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, a 918…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP118

Plasmid

#21671

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP125

Plasmid

#21674

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been completely dele…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only