1. p426 25Q GPD

   Plasmid

   #1181

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

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1. pET42b-BE3

   Plasmid

   #87437

   Purpose

   Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-GGamma11-GFP2

   Plasmid

   #166780

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma11-GFP2 (GNG11 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

   Plasmid

   #165444

   Purpose

   Expresses ACX, rXRCC1 in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rXRCC1 (Apobec1 Rat, Synthetic, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. K9L mutant of H3K9me3 biosensor

   Plasmid

   #120807

   Purpose

   FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   lysine 9 is mutated to leucine 9 on histone H3

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HF-BE3

   Plasmid

   #87439

   Purpose

   Expresses HF-BE3 with C-terminal NLS in mammalian cells

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mammalian codon-optimized Cas9-HF

   Promoter

   CMV

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. B52_puro_gRNA_3418711_KCNB2

   Plasmid

   #197558

   Purpose

   gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

   Depositor

   Darius Bägli

   Insert

   upstream KCNB2 promoter gRNA (Kcnb2 Rat)
   

   Use

   Grna with puromycin selection

   Tags

   puromycin resistance cassette under CMV promotion

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGS_128

   Plasmid

   #160651

   Purpose

   Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e3 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e3 allele

   Promoter

   pZ estradiol inducible promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Circular 200,100 with interspersed loops (GAPDH)

   Plasmid

   #180183

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Circular 200,100 with 8 bp bulge loops (GAPDH)

   Plasmid

   #170121

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGS_101

    Plasmid

    #160541

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGS_107

    Plasmid

    #160650

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGS_129

    Plasmid

    #160652

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    pZ estradiol inducible promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. APOE_pLX307

    Plasmid

    #98317

    Purpose

    Lentiviral expression of APOE

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. S10A mutant of H3S10ph biosensor

    Plasmid

    #120810

    Purpose

    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 10 is mutated to Alanine, and Threonine 3,…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3Basic-ME.2/ApoEpromoter

    Plasmid

    #51436

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    APOE apolipoprotein E (APOE, ME2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The ME.2 enhancer is fused upstream of the ApoE g…

    Promoter

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3Basic_ME.1/ApoEpromoter

    Plasmid

    #51435

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    ME.1 enhancer is fused upstream of ApoE promoter …

    Promoter

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

19. LRP4 (extracellular) scFv [N207/27]

    Plasmid

    #190537

    Purpose

    Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-LRP4 (cytoplasmic) [N164/6.1R]

    Plasmid

    #114542

    Purpose

    Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge