1. p426 25Q GPD

   Plasmid

   #1181

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pGS_101

   Plasmid

   #160541

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GAPDH

   Plasmid

   #155622

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GAPDH (GAPDH Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. APOE_pLX307

   Plasmid

   #98317

   Purpose

   Lentiviral expression of APOE

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   APOE (APOE Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

   Plasmid

   #136652

   Purpose

   Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGS_129

   Plasmid

   #160652

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   pZ estradiol inducible promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGS_128

   Plasmid

   #160651

   Purpose

   Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e3 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e3 allele

   Promoter

   pZ estradiol inducible promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Circular 200,100 with interspersed loops (GAPDH)

   Plasmid

   #180183

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. S10A mutant of H3S10ph biosensor

   Plasmid

   #120810

   Purpose

   FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Serine 10 is mutated to Alanine, and Threonine 3,…

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGS_107

   Plasmid

   #160650

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Circular 200,100 with 8 bp bulge loops (GAPDH)

    Plasmid

    #170121

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LRP4 (extracellular) scFv [N207/27]

    Plasmid

    #190537

    Purpose

    Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-LRP4 (cytoplasmic) [N164/6.1R]

    Plasmid

    #114542

    Purpose

    Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3Basic-ME.2/ApoEpromoter

    Plasmid

    #51436

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    APOE apolipoprotein E (APOE, ME2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The ME.2 enhancer is fused upstream of the ApoE g…

    Promoter

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3Basic_ME.1/ApoEpromoter

    Plasmid

    #51435

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    ME.1 enhancer is fused upstream of ApoE promoter …

    Promoter

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

15. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid

    #114009

    Purpose

    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection

    Depositor

    Pavel Volchkov

    Article

    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126

    Inserts

    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    -, CMV, and endogenous promoter (chicken GAPDH in…

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid

    #136659

    Purpose

    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. apoeb (apolipoprotein Eb)_L (OZ529)

    Plasmid

    #27206

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting apoeb (apoeb Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. apoeb (apolipoprotein Eb)_R (OZ530)

    Plasmid

    #27207

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting apoeb (apoeb Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. Anti-Kv2.2 K+ channel scFv [N372C/51 scFv]

    Plasmid

    #219495

    Purpose

    Mammalian Expression Plasmid of anti-Kv2.2 K+ channel (Rat) scFv. Derived from hybridoma N372C/51.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Kv2.2 K+ channel (Rattus norvegicus) recombinant mouse scFv. (Kcnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Kv2.2 K+ channel scFv [N372B/60]

    Plasmid

    #206740

    Purpose

    Mammalian Expression of Kv2.2 K+ channel scFV. Derived from hybridoma N372B/60 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Kv2.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge