Addgene

We narrowed to 13,821 results for: pET

Showing: 3141 - 3160 of 13821 results

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pETM-60-NusA-HsTNRC6B-UBA_F

Plasmid

#146268

Purpose

Bacterial Expression of HsTNRC6B-UBA

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-UBA (TNRC6B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-HsTNRC6C-UBA_F

Plasmid

#146269

Purpose

Bacterial Expression of HsTNRC6C-UBA

Depositor

Elisa Izaurralde

Insert

HsTNRC6C-UBA (TNRC6C Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmEDC3_331-680_D

Plasmid

#146100

Purpose

Bacterial Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Insert

DmEDC3_331-680 (Edc3 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmTral_395-542_D

Plasmid

#146101

Purpose

Bacterial Expression of DmTral_395-542

Depositor

Elisa Izaurralde

Insert

DmTral_395-542 (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmEDC3_331-680_D

Plasmid

#146102

Purpose

Bacterial Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Insert

DmEDC3_331-680 (Edc3 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmTral_395-542_D

Plasmid

#146103

Purpose

Bacterial Expression of DmTral_395-542

Depositor

Elisa Izaurralde

Insert

DmTral_395-542 (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO1(wt)

Plasmid

#191798

Purpose

Full length wt AtPCO1 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO1

Use

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO2(wt)

Plasmid

#191799

Purpose

Full length wt AtPCO2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO2

Use

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO3(wt)

Plasmid

#191800

Purpose

Full length wt AtPCO3 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO3

Use

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:em-blue-shift.2

Plasmid

#191919

Purpose

Encodes a GFP design, sf:em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:em-blue-shift.2

Use

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)-MutTER

Plasmid

#178035

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted and Changed T(8…

Promoter

T7LacI

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-E67A_rpoA-U

Plasmid

#190959

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-A66S_rpoA-C

Plasmid

#190968

Purpose

Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-A66S_rpoA-U

Plasmid

#190957

Purpose

Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3-A66S_rpoA-U

Plasmid

#190961

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3-E67A_rpoA-U

Plasmid

#190963

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-E67A_rpoA-C

Plasmid

#190972

Purpose

Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 pET21a+

Plasmid

#172321

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229L-pET151

Plasmid

#177879

Purpose

Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229S-pET151

Plasmid

#177878

Purpose

Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Serine (P229S)

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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