Addgene

We narrowed to 14,718 results for: LEA

Showing: 3141 - 3160 of 14718 results

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II 31-52

Plasmid

#35179

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of C-terminal domain repeats 1-30

Promoter

CMV

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Pol-II 1-22

Plasmid

#35178

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of C-terminal domain repeats 23-52

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-supervillin

Plasmid

#13040

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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GPKA

Plasmid

#61091

Purpose

GFP-tagged cAMP-dependent PKA catalytic subunit

Depositor

Steven Green

Article

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pET-PhoCl1-6His

Plasmid

#164033

Purpose

Expressed PhoCl1 in a pET expression vector

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl1

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSQT817

Plasmid

#53373

Purpose

wildtype Cas9 expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pGWB660-CYCD3;1

Plasmid

#140751

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

CaMV 35 promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJY3919

Plasmid

#113332

Purpose

Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTNH3

Plasmid

#110074

Purpose

Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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HS-Par3-GFP

Plasmid

#105947

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Par3

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-600n

Plasmid

#13545

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pKJ237

Plasmid

#212320

Purpose

Express KnFnuc

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ226

Plasmid

#212316

Purpose

Express DpFnuc with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-deltaCKAR

Plasmid

#31526

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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