Addgene

We narrowed to 15,233 results for: LEA

Showing: 3141 - 3160 of 15233 results

Results per page:

Sort results by:

TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Membrane mCherry

Plasmid

#210470

Purpose

Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

Depositor

Amro Hamdoun

Insert

LCK membrane mCherry (LCK A.victoria, Human, Synthetic)

Use

Synthetic Biology; Sea urchin, mammal, zebrafish

Tags

mCherry

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ZipGFP1-10_TEV

Plasmid

#81242

Purpose

Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HS-Par3-GFP

Plasmid

#105947

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Par3

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_g5-HT2h

Plasmid

#208709

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cells

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p15a-AsCas12f-apmR

Plasmid

#171610

Purpose

AsCas12f1-based K. pneumoniae genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TS module

Plasmid

#26021

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Tension Sensor module

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFi-dHdrA

Plasmid

#184907

Purpose

carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cm-sgRNA-dCas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-hBcan

Plasmid

#18966

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Use

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Promoter

Available since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-6

Plasmid

#58782

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-pDisplay-SNAP

Plasmid

#223495

Purpose

Expression of SNAP-tags to outer surface in mammalian cells

Depositor

James Frank

Article

Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

Insert

SNAP-tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP NFAT5 (no EGFP)

Plasmid

#13627

Depositor

Anjana Rao

Article

Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

Insert

NFAT5 (NFAT5 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpaCas12f1-gRNA_MS13

Plasmid

#190877

Purpose

The plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cells

Depositor

Quanjiang Ji

Article

Wang et al Cell Rep. 2022 Sep 27;40(13):111418. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSVK3-Flag-MAN1

Plasmid

#26002

Depositor

Howard Worman

Article

Wu et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1361-7

Insert

LEMD3 LEM domain containing 3 (LEMD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-dCas9-KRAB-gRNA-TRE-blast

Plasmid

#201152

Purpose

Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

Depositor

Moshe Szyf

Inserts

dCas9-KRAB
gRNA-TRE

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mutation

gRNA sequence: TACGTTCTCTATCACTGAT

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lbpro (29-195)

Plasmid

#110759

Purpose

Bacterial expression of Lbpro

Depositor

Tim Skern

Article

Guarne et al EMBO J. 1998 Dec 15;17(24):7469-79. doi:

Insert

Lb pro

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge