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Plasmid#196174PurposeMoClo Level 1 luciferase reporter: proANAC032::LucF::flag::notTDepositorInsertproANAC032::LucF::flag::notT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
nNc-ABE
Plasmid#194095PurposeCMV-TadA 8e-nNcCas9. A-to-G base editing in human cells.DepositorInsertTadA 8e-nNcCas9
UseTagsExpressionMammalianMutationNcCas9 D16APromoterCMVAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_X5C_S277C
Plasmid#190177PurposeFor mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibilityDepositorInsertrat serotonin transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationCys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL5N_LT32-HBB_X1
Plasmid#191520PurposeExpression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left)DepositorInsertZF-DdCBE LT32-HBB X1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:miRFP670-cetn4
Plasmid#192357PurposezfCETN4 tagged with miRFP670, expressed under the UAS promoterDepositorInsertCETN4 (cetn4 Zebrafish)
UseTagsmiRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16-18 E7 Chimera B
Plasmid#191543PurposeProtein expression of HPV16 E7 Chimera BDepositorInsertHPV16-18 E7
UseRetroviralTagsFlagHAExpressionMutationCR1 CR2 HPV18 E7, C-terminus HPV16 E7PromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16-18 E7 Chimera A
Plasmid#191542PurposeProtein expression of HPV16 E7 Chimera ADepositorInsertHPV16-18 E7
UseRetroviralTagsFlagHAExpressionMutationCR1 CR2 HPV16 E7, C-terminus HPV18 E7PromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16-18 E7 Chimera C
Plasmid#191544PurposeProtein expression of HPV16 E7 Chimera CDepositorInsertHPV16-18 E7
UseRetroviralTagsFlagHAExpressionMutationCR1 HPV18 E7, CR2 C-terminus HPV16 E7PromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2139
Plasmid#162132PurposeExpression and Complementation plasmid for MlaD TM swap with LptC TMDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-(6xHis-MlaE)-MlaD(TMdeltaLptC TM)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2138
Plasmid#162133PurposeExpression and Complementation plasmid for MlaD TM swap with LetB TMDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-(6xHis-MlaE)-MlaD(TMdeltaLetB TM)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL1198
Plasmid#162134PurposeExpression and complementation plasmid for MlaFEDCB, with N-terminal his tag on MlaEDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-(6xHis-MlaD)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2093
Plasmid#162135PurposeExpression and Complementation plasmid for MlaE IF1 1-15 aa deletionDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE(delta1-15)-(6xHis-MlaD)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2132
Plasmid#162136PurposeExpression and Complementation plasmid for MlaE IF1 1-25 aa deletionDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE(delta1-25)-(6xHis-MlaD)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2092
Plasmid#162137PurposeExpression and Complementation plasmid for MlaE IF1 1-30 aa deletionDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE(delta1-30)-(6xHis-MlaD)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2133
Plasmid#162138PurposeExpression and Complementation plasmid for MlaE IF1 1-39 aa deletionDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE(delta1-39)-(6xHis-MlaD)-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL193
Plasmid#180471PurposeFor expression of Cas9 and gRNAs, multiple gRNAs cloning vectorDepositorTypeEmpty backboneUseCRISPRTagsCas9ExpressionPlantMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Isce-Tol2-Tn5-neo
Plasmid#176619Purposefor generating PCR templates with Tol2 repeats and Isce sites for BAC recombinationDepositorInsertI-SceI sites, Tol2 repeats and Tn5-neo cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH010
Plasmid#157645PurposesMHT-113 fused non-dimerizing GRX2-C37A mutantDepositorInsertssMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH011
Plasmid#157646PurposesMHT-113 fused to FKBP/FRBDepositorInsertssMHT(1-113)-FKBP
FRB-sMHT(114-230)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH012
Plasmid#157647PurposeConstitutive sMHT-113-SYNZIP expressionDepositorInsertssMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only