Addgene

We narrowed to 14,718 results for: LEA

Showing: 3101 - 3120 of 14718 results

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pRS423-TyrSgH

Plasmid

#163973

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rBcan

Plasmid

#18958

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican (Bcan Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pGP-FB-orig BA

Plasmid

#13420

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

orig BA

Use

Tags

Gal11P

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-RalGDS

Plasmid

#118315

Purpose

Expression of human RalGDS

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RalGDS (RALGDS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

fragment of human RalGDS 203aa

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Gal4-PGC1 alpha

Plasmid

#8892

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

Gal4 DBD

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Use

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-wt

Plasmid

#33030

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMJ240: pRS426-His-MBP-MmeFz2_Native

Plasmid

#205255

Purpose

S. cerevisiae expression vector for MmeFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC-e5

Plasmid

#120343

Purpose

pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

chr8:130594100-130596043 (hg19)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

MYC

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC-e4

Plasmid

#120342

Purpose

pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

chr8:129060477-129062593 (hg19)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

MYC

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f-2NLS

Plasmid

#171613

Purpose

NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

NLS-AsCas12f1-NLS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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PSuperPuroRetro-1660(hPot1)

Plasmid

#11135

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pSYC-97

Plasmid

#31565

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

pCS4+-NLS-EGFP-P2A-mCherry-CAAX

Use

Tags

Expression

Mutation

Promoter

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Article

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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