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Plasmid#138129PurposeExpresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmClover3-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mucin MUC1-5TR-pET15b
Plasmid#174694PurposePlasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)DepositorInsertMUC1-TR
UseTagshexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
RINS1(mut)
Plasmid#107291PurposePlasmid for the expression of the inactive mutant of RINS1 sensorDepositorInsertRINS1(mut)
UseTagssfGFPExpressionMammalianMutationtwo Arg/Ser mutations at the beginning and in the…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEX-GECO1
Plasmid#32443PurposeMammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEX-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Syk Biosensor
Plasmid#125729PurposeFRET biosensor for studying SYK activities in cellsDepositorInsertSYK biosensor (ZAP70 Synthetic)
UseTags6xHistine Tag at the N-terminal.ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p4B5-EGFP
Plasmid#140324PurposeDetection of Dengue virus infection in live cellsDepositorInsertDENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
UseTagseGFPExpressionMammalianMutation2 conservative n.t. differences near 5' end …PromoterCMVAvailable sinceMay 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt(R23/25A)
Plasmid#181835PurposePhospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.DepositorInsertLyn-dInPAkt
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationArginines 23 and 25 in PH domain mutated to alani…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)
Plasmid#128607PurposeExpresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC.c - Display - CyRFP1
Plasmid#193945PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC.c
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-cerulean
Plasmid#53748PurposeContains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.DepositorInserthistone H2B (H2BC21 Human)
UseTagsCeruleanExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mVenus
Plasmid#107606PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tagDepositorInsertMS2-mVenus
UseSynthetic BiologyTagsV5 and mVenusExpressionMutationMS2: V75E/A81GPromoterhuman UBCAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCardinal2
Plasmid#52631Purposebacterial expression vector with far-red FP mCardinal2DepositorInsertmCardinal2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag
Plasmid#132400Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121APromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorInsertTubulin
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro BRIT1
Plasmid#16205DepositorInsertBRIT1 (MCPH1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)
Plasmid#185802Purposeone plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdKatushka2-VASP-5
Plasmid#56049PurposeLocalization: Actin/Focal Adhesions, Excitation: 586, Emission: 632DepositorInsertVASP
UseTagstdKatushka2ExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mPlum-Mito-3
Plasmid#55988PurposeLocalization: Mitochondria, Excitation: 590, Emission: 649DepositorInsertMito (COX8A Human)
UseTagsmPlumExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER-ABKAR
Plasmid#61508PurposeExpression of AMPK biosensor targeted to the endoplasmic reticulumDepositorInsertAMPK Biosensor
UseTagsER targeting sequence from CYP450 2C1 (see commen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB16 (pmyo-3::QuasAr)
Plasmid#130272PurposeExpresses the voltage sensor QuasAr in BWMs of C. elegans.DepositorInsertQuasAr
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only