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Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE254 pCDNA3.1(zeo)-hDsh3-497-716
Plasmid#16755DepositorInsertFLAG-497-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 497-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR173
Plasmid#106058PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)DepositorInsertOpto-GPR173 (GPR173 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR31
Plasmid#106014PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)DepositorInsertOpto-GPR31 (GPR31 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-G13-G12
Plasmid#25742PurposeLentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.DepositorInsertG alpha 13 and 12 miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4C (216-233) (pGEX2T(TEV))
Plasmid#80635PurposeResidues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202DepositorInsertCHMP4C (CHMP4C Human)
UseTagsGSTExpressionBacterialMutationResidues 216-233PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F3UXG6
Plasmid#103108PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBRCA1 (BRCA1 Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-C7TEQ6
Plasmid#103092PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR25
Plasmid#106011PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)DepositorInsertOpto-GPR25 (GPR25 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER
Plasmid#16753DepositorInsertFLAG-hDsh3-deltaCTER (DVL3 Human)
UseTagsflagExpressionMammalianMutationdelta CTERPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR-mDIXDC1 WT
Plasmid#61216PurposeEntry clone encoding long isoform of mouse DIXDC1DepositorInsertDIXDC1 (Dixdc1 Mouse)
UseDonr clone (gateway)TagsExpressionMutationPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-4MS2-xrRNA-4H
Plasmid#108371PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Opto-GPR119
Plasmid#106040PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)DepositorInsertOpto-GPR119 (GPR119 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMXs-hs-3xHA-C12AC15A-LIN-41
Plasmid#52725Purposeretroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged cysteines 12 and 15 to alaninesPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR21
Plasmid#106009PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR21; Opto-GPR21; A11)DepositorInsertOpto-GPR21 (GPR21 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR1
Plasmid#105999PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR1; Opto-GPR1; A1)DepositorInsertOpto-GPR1 (GPR1 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D4J3S7
Plasmid#103096PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD4J3S7(Cas9 coding gene from Coprococcus catus GD/7)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only