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Showing: 3081 - 3100 of 3974 results

  4. M13cp-dg2 hp

    Plasmid
    #218094
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218094-plasmid-map-sequence-id-437754

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  13. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al bioRxiv. (PrePrint)
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  17. M13cp-dg1 hp

    Plasmid
    #218093
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218093-plasmid-map-sequence-id-432465

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  18. LacI pm

    Plasmid
    #218097
    Purpose
    Phagemid that expresses LacI but no phage proteins, used as a control
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218097-plasmid-map-sequence-id-432441

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  19. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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  20. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

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Showing: 3081 - 3100 of 3974 results