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Plasmid#61272PurposeAlso referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 ICv-6MT
Plasmid#41730DepositorInsertNotch-1 Intracellular Domain (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 protein beginning at Vali…PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x4
Plasmid#58768PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HIRA-polyA
Plasmid#59781PurposeExpress H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertHIRA
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
UseTags3xFLAG-tag and His14-tagExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e3
Plasmid#120341PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:129056967-129058438 (hg19)
UseLuciferaseTagsExpressionMammalianMutationPromoterMYCAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG2-wPRE-pA
Plasmid#60489PurposeeGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)DepositorInsertIRES-eGFP; wPRE; BGH-pA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJ106: pRS426-His-MBP-NlovFz2_Native
Plasmid#205253PurposeS. cerevisiae expression vector for NlovFz2 native guideDepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Membrane mCherry
Plasmid#210470PurposePlasma membrane localizing mCherry using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mCherry (LCK Human, A.victoria, Synthetic)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCherryExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ45: pRS426-His-MBP-SpuFz1_Native
Plasmid#205252PurposeS. cerevisiae expression vector for SpuFz1 native guideDepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH625_pJH_LldR_PlldR-sfGFP.0TAG
Plasmid#163724PurposeLactate-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB8_4-3i-ATP8_v8_AGKS
Plasmid#191513PurposeExpression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)DepositorInsertv8 ZF-DdCBE 4-3i-ATP8 AGKS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB5_R8-3i-ATP8_v8_AGKS
Plasmid#191510PurposeExpression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)DepositorInsertv8 ZF-DdCBE R8-3i-ATP8 AGKS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
wg186Fluc
Plasmid#177436PurposeIn vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.DepositorInsertFirefly luciferase
UseLuciferase; For in vitro transcriptionTagsNoneExpressionMutationPromoterT7Available sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-sgTel
Plasmid#122659PurposeExpresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection markerDepositorInsertBFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-GFP-ECL-3TM-tevs-KKMP V3
Plasmid#179640PurposeTEVP-inducible membrane-bound GFP RELEASE constructDepositorInsertMembrane-bound GFP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFP
Plasmid#175593PurposeLac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.DepositorInsertCFP (cerulean)
UseTagsExpressionMutationAn improved monomeric CFP. See doi: 10.1038/nbt945PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Cherry-N1-PRKAG1
Plasmid#30308DepositorInsertAMP-activated Protein Kinase γ1 (PRKAA1 Human)
UseTagsFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMX/cyclinB1 (17)
Plasmid#26060DepositorInsertCyclin B1 (CCNB1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pColE1-ISceI
Plasmid#179578PurposeExpresses I-SceI from an arabinose promoterDepositorInsertI-SceI (I-SceI Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only