Addgene

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pMM0286

Plasmid

#128974

Purpose

Reporter plasmid, pZF(3oBS)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3oBS)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0619

Plasmid

#128994

Purpose

Spacer cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-Spacer-ConRE-AmpR-Col-E1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0289

Plasmid

#128977

Purpose

Reporter plasmid, pZF(4BSc)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(4BSc)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0285

Plasmid

#128973

Purpose

Reporter plasmid, pZF(1BS)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(1BS)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103 DYSTROPHIN WW domain

Plasmid

#104268

Purpose

Bacterial expression of WW domain from DYSTROPHIN

Depositor

Sachdev Sidhu, Marius Sudol

Insert

DYSTROPHIN WW domain (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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TNFa-GFP

Plasmid

#163660

Purpose

Expresses GFP tagged TNFa in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

tumor necrosis factor (TNF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMM0553

Plasmid

#128989

Purpose

LEU2 integration vector of pZF(3BS)-mRUBY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-D91G

Plasmid

#47646

Purpose

pAC-EsaR with a a272g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-D91G

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Aspartic acid 91 to Glycine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pMM0530

Plasmid

#128987

Purpose

Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2PHR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pNM3689

Plasmid

#154111

Purpose

miniMos single component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Tags

Expression

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3649

Plasmid

#154110

Purpose

miniMos dual component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Tags

Expression

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMM0623

Plasmid

#128998

Purpose

pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSL6 SMR1747

Plasmid

#28029

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutS (mutS E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pCR.MCV.Ori339 (589)

Plasmid

#28192

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCV replication region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
VPS35 shRNA1

Plasmid

#163858

Purpose

shRNA targeting the coding sequences of VPS35

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

VPS35 retromer complex component (VPS35 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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