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Plasmid#103119PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiR-96 cluster promoter
Plasmid#62517PurposemicroRNA 183-96-182 cluster promoter luciferase plasmidDepositorInsertpromoter of microRNA-183-96-182 cluster
UseTagsRenSP (optimized renilla luciferase gene)ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.2-coHPDL WT 3xFlag-V5
Plasmid#174129PurposeExpresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimizedPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A0LWB3
Plasmid#103070PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBabepuro-Willin
Plasmid#40884DepositorInsertWillin (FRMD6 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
XE252 pCDNA3.1(zeo)-hDsh3-333-716
Plasmid#16757DepositorInsertFLAG-333-716-Dsh3 (DVL3 Human)
UseTagsFlagExpressionMammalianMutationaa 333-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X
Plasmid#16759DepositorInsertVSV-KLHL12-Q405X (KLHL12 Human)
UseTagsvsvExpressionMammalianMutationQ405STOPPromoterAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ
Plasmid#16754DepositorInsertFLAG-hDsh3-trunc before PDZ (DVL3 Human)
UseTagsflagExpressionMammalianMutationtruncated before PDZPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4-K129R (GST)
Plasmid#21564DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
UseTagsGSTExpressionMammalianMutationLysine 129 to argininePromoterAvailable sinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras 12V (40C)
Plasmid#12590DepositorInsertHRas 12V 40C (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V, 40CPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
XE253 pCDNA3.1(zeo)-hDsh3-82-716
Plasmid#16756DepositorInsertFLAG-82-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 82-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras 12V (35S)
Plasmid#12588DepositorInsertHRas 12V 35S (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V, 35SPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEN_TGmiR_Gb2-K
Plasmid#25766PurposeEntry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.DepositorInsertGb2 miR-shRNA (Gnb2 Mouse)
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6I4Q5
Plasmid#103101PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaCoiledCoil-LIN-41
Plasmid#52720Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 328–447PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4MS2-4H
Plasmid#108370PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only