-
Plasmid#15096DepositorInsertIRAK1 3'UTR (IRAK1 Human)
UseLuciferaseTagsExpressionMammalianMutation4-bp substitutions in the miR-146 target sitesPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-ltDHFR(DD)
Plasmid#47080PurposeecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegansDepositorInsertYFP-ltDHFR(DD)
UseRetroviralTagsHA and YFPExpressionMutationamino acids mutations: R12H, N37D, N59D, G67S, D1…PromoterLTRAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 stuffer v3
Plasmid#158046Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS18
Plasmid#158787PurposeRibosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegansDepositorInsertPtag-168::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-HA-UBE3BΔHECT-IRES-Puro
Plasmid#107347PurposeExpresses HA tagged UBE3B deletion of HECT domain in mammalian cells.DepositorInsertUBE3BΔHECT (UBE3A Human)
UseLentiviralTagsHAExpressionMammalianMutationdeletion HECT domain (757-end)PromoterCMVAvailable sinceJune 26, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAH18
Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNAAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNH12 (pmyo-2::MacQ::mCitrine)
Plasmid#130274PurposeExpresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.DepositorInsertMacQ::mCitrine
UseTagsmCitrineExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ANKRD24-TwinStrepII
Plasmid#83190PurposeExpression of tagged protein in mammalian cellsDepositorInsertANKRD24 (Ankrd24 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-0.27kb-EGFP
Plasmid#153185PurposeTruncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7053 pHAGE-P-CMVt-N-HA-GAW-BICD2
Plasmid#100143PurposeExpresses BICD2DepositorInsertBICD2 (BICD2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSVE Grb2 myc 49L/203R
Plasmid#26087DepositorInsertGrb2 49L/203R (GRB2 Human)
UseTagsmycExpressionMammalianMutation49L/203RPromoterAvailable sinceSept. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
AID-BFP-loxP_myo2_neoR
Plasmid#194054PurposeDual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genomeDepositorInsertIAA17 (AXR3 Mustard Weed)
UseTagsbfpExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p7127 pHAGE-P-CMVt-N-HA-GAW-FTL
Plasmid#100153PurposeExpresses FTLDepositorInsertFTL (FTL Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain
Plasmid#183397PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI K270A
Plasmid#167290PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationK270APromoterSFFVAvailable sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v4
Plasmid#158032Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV-GSDMD_C192A-Myc
Plasmid#201168PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycExpressionMutationC192APromoterMESVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-0.66kb-EGFP
Plasmid#153165PurposeTruncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UBE3B-BirA-Puro
Plasmid#107352PurposeExpresses UBE3B fused to BirA in mammalian cells.DepositorInsertUBE3B-BirA (UBE3B Human)
UseLentiviralTagsBiotin ligaseExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-CMV-cGASΔN-HA (murine)
Plasmid#130915PurposeExpresses murine cGASΔN (aa148-507)-HA; Puromycin selection markerDepositorInsertcGASΔN murine
UseTagsHAExpressionMammalianMutationmurine cGAS catalytic domain (aa148-507)PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only