-
Plasmid#76584Purpose3rd generation lentiviral gRNA plasmid targeting human AK3DepositorInsertAK3 (AK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AK3 gRNA (BRDN0001148568)
Plasmid#76585Purpose3rd generation lentiviral gRNA plasmid targeting human AK3DepositorInsertAK3 (AK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BMPR1A gRNA (BRDN0001147849)
Plasmid#77959Purpose3rd generation lentiviral gRNA plasmid targeting human BMPR1ADepositorInsertBMPR1A (BMPR1A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA
Plasmid#187576PurposeExpress FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cellsDepositorInsertLMNA (LMNA Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AIP(WT)-mChF-giantin
Plasmid#61523PurposeExpression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIPDepositorUseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry_Atg4BC74A
Plasmid#161735PurposeDoxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagyDepositorInsertAtg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsmStrawberryExpressionMammalianMutation220-222 TGC (Cys) is changed to GCA (Ala)PromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3M14nes
Plasmid#157855PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertUseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) SDC3
Plasmid#107501PurposeExpresses SDC3, puromycin resistantDepositorInsertSDC3 (codon optimized) (SDC3 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SIP1_WT
Plasmid#82241PurposeGateway Donor vector containing GEMIN2 ,part of the Target Accelerator Plasmid Collection.DepositorInsertGEMIN2 (GEMIN2 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSC-ISL1-T2A-LHX3
Plasmid#90215PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha/TRE3GAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-PROGERIN
Plasmid#140189PurposeOverexpression of Progerin with a GFP fusion protein, under human synapsin promotorDepositorInsertProgerin (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Lamin A wildtype
Plasmid#118709PurposeLentiviral TET-ON inducible GFP-Lamin A wildtypeDepositorInsertGFP-Lamin A human wildtype (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationPromoterCMV TRE3G (TET-ON)Available sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgCnr1
Plasmid#209196PurposeMutagenesis of Cnr1DepositorInsertCnr1 (Cnr1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterCMVAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-HAPLN1
Plasmid#200777PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationPromoterSynapsinAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LTA-Fc(DAPA)-AviTag-6xHis
Plasmid#156568PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertLTA (LTA Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-∆Hapln1
Plasmid#200778PurposeExpresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationdeleted lectican binding domain (aminoacids 40-15…PromoterSynapsinAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR2
Plasmid#116741PurposeLentiviral expression of FGFR2DepositorInsertFGFR2 (FGFR2 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry
Plasmid#172627PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only