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Plasmid#121828PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7) (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KH001: pMVP (L3-L2) V5 epitope tag + WPRE
Plasmid#121762PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertV5 epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP L1-R5
Plasmid#62156PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV eGFP R4-R3
Plasmid#62141PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloningDepositorInserteGFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold
Plasmid#121812PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SFFV L1-R5
Plasmid#62097PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 L1-R5
Plasmid#62111PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV tdTomato R4-R3
Plasmid#62151PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloningDepositorInserttdTomato
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV/TO L1-R5
Plasmid#62099PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1-pCR8-dCas9VP48
Plasmid#48214PurposedCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP48 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP48ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV eGFP L1-R5
Plasmid#62142PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloningDepositorInserteGFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
LA102: pMVP (L3-L2) P2A-eYFP + WPRE
Plasmid#121778PurposepMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eYFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP L5-L2
Plasmid#62157PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 PuroR L3-L2
Plasmid#62164PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloningDepositorInsertPuromycin
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV/TO R4-R3
Plasmid#62100PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK L1-L4
Plasmid#62110PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
LA301: pMVP (L3-L2) P2A-Hygro + WPRE
Plasmid#121787PurposepMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectorsDepositorInsertP2A-Hygro + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV mCherry R4-R3
Plasmid#62147PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloningDepositorInsertmCherry
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 LacZ L5-L2
Plasmid#62167PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and LacZ module. Compatible with MultiSite Gateway cloningDepositorInsertLacZ
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 tdTomato L5-L2
Plasmid#62154PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloningDepositorInserttdTomato
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only