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Plasmid#120943PurposeMoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.DepositorInsertPlacO (Tim Lu, Matt Bennett, etc)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-Q249A
Plasmid#222216PurposeExpress DDX53 mutant Q249A protein in bacteriaDepositorInsertDDX53_Q249A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
3C5I
Plasmid#60118PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPKN-PF14_0020:MAC029-F01:C34272
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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RV-Syn-Dio-yfp_2a_elfn1
Plasmid#98177PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-elfn1DepositorInsertElfn1 (Elfn1 Mouse)
UseRetroviralTagsExpressionMutationPromoterSynAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]
Plasmid#86456PurposeLox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX160
Plasmid#126378PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf07272g01001.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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IDG_CACNA2D3_OE_1
Plasmid#161683PurposeOverexpresses CACNA2D3DepositorInsertCACNA2D3 (CACNA2D3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
STK10 (STK10A-c043)
Plasmid#110255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSTK10 (STK10 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSN6
Plasmid#131318PurposeIntegrating the full-length tetO array (from pLAU44) near the his-3 gene in N. crassaDepositorInsertFull-length tetO array (XbaI-NheI fragment of pLAU44)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPM_Z
Plasmid#148126PurposeMammalian Expression of HshnRNPMDepositorInsertHshnRNPM (HNRNPM Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequenceof t…PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSN7A
Plasmid#131319PurposeExpressing synthetic TetR-GFP protein in N. crassa (from the his-3 locus)DepositorInsertTetR-GFP
UseUnspecifiedTagsEGFPExpressionMutationPromoterTrpC (from pCSN44)Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOP-H2B-mCherry-DD
Plasmid#96894PurposeReporter to monitor translation of 5'TOP mRNADepositorInsertTOP-H2B-mCherry-DD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
TOP-H2B-YFP-DD
Plasmid#96891PurposeReporter to monitor translation of 5'TOP mRNADepositorInsertTOP-H2B-YFP-DD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-WGA-mCherry
Plasmid#224119PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.DepositorInsertWGA-mCherry
UseAAVTagsWGA and mCherryExpressionMammalianMutationAll mutations related to the WGA in this construc…PromoterCAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML33
Plasmid#206996PurposeContains OsTiR1 ORF and LoxP and Lox2272 sites.DepositorInsertOsTiR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPM_243-615_Z
Plasmid#148127PurposeMammalian Expression of HshnRNPM_243-615DepositorInsertHshnRNPM_243-615 (HNRNPM Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence of t…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only