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Plasmid#134863PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertH2B-emiRFP703
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsEYFP and SNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-GFP-RGG
Plasmid#124948PurposeExpression of RGG-GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsEGFP and HisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-EGFP-Donor
Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorInsertEGFP
UseIntron tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-EBFP-tTAn
Plasmid#172122PurposeConstitutive expression of EBFP and rtTA-InteinNDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP2-Flag-His-WT
Plasmid#111574PurposeExpresses full length PARP2 in Sf9 (insect) cellsDepositorInsertPARP2 (PARP2 Human)
UseTagsC-terminal 6xHis and C-terminal FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a)
Plasmid#79672PurposeCre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP (Scn9a jellyfish, Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6
Plasmid#190162PurposeExpresses 24 Broccoli-tagged beta-actin mRNA in mammalian cellsDepositorInsertmcherry_(3'UTR, No Actin)_24Broccoli
UseTags24×BroccoliExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
DD-DamWT-IRES2-mCherry
Plasmid#159602PurposeShield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam.DepositorInsertDD-DamWT-IRES2-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 MT
Plasmid#78261PurposepsiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CTCC to GA…PromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-RNF8
Plasmid#99396PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rap1A FLARE
Plasmid#134926PurposeRap1A biosensorDepositorInsertRap1A, mCerulean3, RaIGDS RBD, YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pyc126m
Plasmid#158777Purposeribosome-tagged GCaMP for soma localizationDepositorInsertGCaMP6m-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-PGK-EiP
Plasmid#21312PurposeControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFU-GW-sMYH6-mNeon-TNNT2-mScarlet
Plasmid#170712Purposedual fluorescent reporter for MYH6 (short) and TNNT2 promotersDepositorInsertsmNeonGreen
mScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterTNNT2 and aMHCAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(Hygro) TagBFP-hNucleolin
Plasmid#182592Purposeexpresses bfp-hNucleolin in mammalian cellsDepositorInserthNucleolin (NCL Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ChromosomesMembranesandCytoskeleton
Plasmid#62450PurposeMXS_chaining vector with 4 cassettesDepositorInsertsCMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only