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Plasmid#221695PurposeMammalian expression of FLAG-tagged human RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN554
Plasmid#177361PurposeExpresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tagDepositorInsertKIF1A (KIF1A Human)
UseTagsLeucine zipper, StrepII tag, and mScarletExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-HOXD13(+10A)-IDR
Plasmid#145257PurposeExpresses fusion of ECFP-LacI and HOXD13+10A IDRDepositorInsertHOXD13(+10A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-HOXD13(+7A)-IDR
Plasmid#145256PurposeExpresses fusion of ECFP-LacI and HOXD13+7A IDRDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-340-5p.sensor.PGK.dNGFR.WPRE
Plasmid#85919PurposeSensor PlasmidDepositorInsertsUseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-Ins2-842
Plasmid#53969Purpose842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assaysDepositorInsertmouse Ins2 gene partial (Ins2 Mouse)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
BM00654
Plasmid#155339PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorUseTagsF2AExpressionMammalianMutationPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRha-ABE8e-SpCas9-NG
Plasmid#201190PurposeExpresses the base editor ABE8e-SpCas9-NG in bacterial cellsDepositorInsertABE8e-SpCas9-NG
UseTags8xHIS and BP-NLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BM00369
Plasmid#155338PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3/hArf6(WT)-EGFP
Plasmid#79423PurposeExpresses C-terminally EGFP-tagged Arf6(WT) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
tdEos-LaminB1-10
Plasmid#57632PurposeLocalization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertLaminB1 (LMNB1 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-Golgi-7
Plasmid#57619PurposeLocalization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertGolgi (B4GALT1 Human)
UseTagstdEosExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Mito-7
Plasmid#57644PurposeLocalization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertMito (COX8A Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag‐mTim3 (del.Cyto)
Plasmid#49209Purposeexpresses Flag tagged mouse Tim3 fragment (no cytosolic domain)DepositorInsertTim3 (del.Cyto) (Havcr2 Mouse)
UseTagsCD8 signal sequence and FLAGExpressionMammalianMutationcytosolic domain deleted, no Tim3 signal sequence…PromoterEf1aAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Actin-C-18
Plasmid#57571PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag‐mTim3 (T2 truncation)
Plasmid#49211Purposeexpresses Flag tagged mouse Tim3 (T2 truncation)DepositorInsertTim3 T2 truncation (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationT2 truncation ( lacks all but one tyrosine, which…PromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only