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Plasmid#186006PurposeExpresses myc-tagged BioID2 fused to SARS-CoV-2 NSP12DepositorInsertSARS-CoV-2 NSP12 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and mycExpressionMammalianMutationPromoterLTRAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSP15-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186009PurposeExpresses SARS-CoV-2 NSP15 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP15 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSP11-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186005PurposeExpresses SARS-CoV-2 NSP11 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP11 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPRBSAAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1047-Tier1-PhCMV-YPet-p2A-PuroR
Plasmid#169498PurposeTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).DepositorInsertPCMV-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
BioID2-1x-ORF9c pBabe-puro (SARS-CoV-2)
Plasmid#185994PurposeExpresses myc-tagged BioID2 fused to SARS-CoV-2 ORF9cDepositorInsertSARS-CoV-2 ORF9c
UseRetroviralTagsBioID2 and mycExpressionMammalianMutationPromoterLTRAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
ORF6-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#185990PurposeExpresses SARS-CoV-2 ORF6 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 ORF6 (ORF6 SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-3xFlag-Neurog1-attB53
Plasmid#183613PurposeRMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertNeurog1 (Neurog1 Mouse)
UseRmceTags3xFlagExpressionMammalianMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-61-SFFV-T2A-PuroR-polyA-R11a
Plasmid#179892PurposeDonor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock outDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-62-SFFV-T2A-PuroR-polyA-R11b
Plasmid#179893PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro
Plasmid#166133PurposeThis plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting cldn1 gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro_TtoC and hairpin extension
Plasmid#167927PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(V303R)-IRES-puro
Plasmid#128659Purposemammalian expression of Flag-Polß(V303R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationV303RPromoterCMVAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165888PurposePuromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro
Plasmid#172641PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPro287AlaPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND1(T286A)-Puro
Plasmid#172642PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationThr286AlaPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro
Plasmid#172633PurposeRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseRetroviralTags2xFLAG-2xSTREPExpressionMammalianMutationThr286AlaPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro
Plasmid#172617PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPro284LeuPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro
Plasmid#172624PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPro281LeuPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-N17-puro
Plasmid#156169PurposeLentiviral vector for expression of flag-RAP1B-N17, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only