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Plasmid#155321PurposeConstitutive expression of E-PIF6(1-100) in plantsDepositorInsertPCaMV35S-E-PIF6(1-100)-NLS-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF394
Plasmid#155333PurposeExpression of PULSE-controlled LFY-VP16-2A-RLucDepositorInsert(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
UseSynthetic Biology; Eukaryotic expressionTagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF417
Plasmid#155335PurposeConstitutive expression LFY-VP16-2A-RLuc in plantsDepositorInsertPCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-7-iRFP670
Plasmid#103032PurposeExpression of iRFP670-tagged Lifeact with G418 selection in mammalian cellsDepositorInsertLifeact
UseTagsiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1
Plasmid#71115Purposemammalian expression of mCherry-LacR-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TPI1_sgRNA1
Plasmid#201626PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTPI1 (TPI1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TPI1_sgRNA2
Plasmid#201627PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTPI1 (TPI1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-ABE(7.10)
Plasmid#108387PurposeMammalian expression vector for xCas9(3.6)-ABEDepositorInsertxCas9(3.6)-ABE(7.10)
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin1 isoform1
Plasmid#72594Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN1 (SESN1 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-G SCT-T2A-GFP
Plasmid#205462Purposelentiviral plasmid for expression of HLA-G SCTDepositorInsertHLA-G SCT (HLA-G Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-TRE-AFOS
Plasmid#197079PurposeA doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.DepositorInsertAFOS (Fos Mouse)
UseLentiviralTagsFlag and T7ExpressionMutationPromoterTREAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His
Plasmid#83453PurposeFull-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)DepositorInsertpolycystin-1 (Pkd1 Mouse)
UseTags2xMyc, 3xFlag, and 6xHisExpressionMammalianMutationS4144DPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK14_HUMAN_D0
Plasmid#79680PurposeThis plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK14
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PGAM1_sgRNA1
Plasmid#201614PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGAM1 (PGAM1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn-HpaBC
Plasmid#100949PurposeExpression of RgTALsyn, HpaB, and HpaC from inducible T7 promoterDepositorInsertsRgTALsyn
HpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf37
Plasmid#12725PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf37
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf48
Plasmid#12736PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf48
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-KPTN
Plasmid#87042Purposemammalian expression of KPTNDepositorInsertKPTN (KPTN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin3
Plasmid#72592Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN3 (SESN3 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
dxCas(3.6)-VPR
Plasmid#108388PurposeMammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminusDepositorInsertdxCas(3.6)-VPR
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only