Addgene

We narrowed to 34,878 results for: kan

Showing: 3001 - 3020 of 34878 results

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pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toru Nakano, Toshinobu Nakamura

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303/7A

Plasmid

#118365

Purpose

This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A and S307A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSHUT4-eYFP

Plasmid

#133094

Purpose

Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

Depositor

Jens Laurids Sørensen

Article

Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

Insert

enhanced yellow flourescent protein

Use

Fungal expression

Tags

Expression

Mutation

Promoter

TEF-1α

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Tags

Expression

Mutation

T301N/N323S

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-HOXD13wt-IDR

Plasmid

#145255

Purpose

Expresses fusion of ECFP-LacI and HOXD13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-7

Plasmid

#57688

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vimentin (VIM Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMVA2

Plasmid

#119951

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Use

Tags

Expression

Bacterial

Mutation

A110G

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

Plasmid

#176535

Purpose

Non-protein coding locus components from CRISPR-associated IscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Sxl].1046B

Plasmid

#112692

Purpose

express two gRNA targeting bTub & Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf5(WT)-EGFP

Plasmid

#79534

Purpose

Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a-Q604L

Plasmid

#79595

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Q604L

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-T559N

Plasmid

#79594

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

T559N

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCR2.1-INS-904

Plasmid

#53970

Purpose

904 bp insert from human INS gene used as a control for methylation-specific PCR assays

Depositor

Kevin Ferreri

Article

Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

Insert

human INS gene partial (INS Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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tdEos-Keratin-17

Plasmid

#57629

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Keratin (KRT18 Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV-FLAG-MAD2-RQ

Plasmid

#221691

Purpose

Mammalian expression of FLAG-tagged human MAD2 (R133E/Q134A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Use

Tags

Expression

Mammalian

Mutation

R133E, Q134A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-MAD2

Plasmid

#221690

Purpose

Mammalian expression of FLAG-tagged human MAD2

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-p31(comet)

Plasmid

#221692

Purpose

Mammalian expression of FLAG-tagged human p31(comet)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-p31(comet)-QF

Plasmid

#221693

Purpose

Mammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Use

Tags

Expression

Mammalian

Mutation

Q83A, F191A

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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