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Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mNeon-ACTB
Plasmid#207751PurposeDonor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS140
Plasmid#197141PurposescCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS191
Plasmid#197140PurposescCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterEF1aAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNM1325
Plasmid#197144PurposeCAGp-Target-AID-2A-BlastR for PiggyBac genomic integrationDepositorInsertSpCas9-PmCDA1-UGI
UseCRISPRTagsExpressionMammalianMutationSpCas9(D10A)PromoterCAGAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-146
Plasmid#197136PurposeCloneSelect Indel reporter encoding BC-del1 barcodeDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-196
Plasmid#197138PurposeCloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-117
Plasmid#197134PurposeCloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-218
Plasmid#197139PurposeAll-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unitDepositorTypeEmpty backboneUseCRISPRTagsSpCas9-PmCDA1-UGIExpressionMammalianMutationSpCas9(D10A)PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-060
Plasmid#197133PurposeCRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expressionDepositorTypeEmpty backboneUseLentiviralTagsEGFP and mCherryExpressionMammalianMutationMCP(N55K)PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-193
Plasmid#197137PurposeCloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationmCherry(M1V/M9A)PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-119
Plasmid#197135PurposeCloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES
Plasmid#182433PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-FPPS
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (M)
Plasmid#182435PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (G)
Plasmid#182477PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase
Plasmid#191481PurposeLuciferase reporter for SARS-CoV-2 5' UTR ΔSL1DepositorInsertSARS-CoV-2 5' UTR ΔSL1
UseLentiviral and LuciferaseTagsExpressionMutationMissing the stem-loop 1 region of SARS-CoV-2 5…PromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
Plasmid#190755PurposeExpression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.DepositorUseTagsmCherry2 and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +D122H
Plasmid#187426PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorUseTagsExpressionInsectMutationchanged aspartic acid at 129 for histidinePromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only