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Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorInsertEGFP
UseIntron tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS
Plasmid#191367PurposeOverexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dMisCas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
Plasmid#129632PurposeVisualization of Akt and ERK activityDepositorInsertPB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_post
Plasmid#119736PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA
UseAAVTagsFLAGExpressionMammalianMutationPromoterhSyn-1Available sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag
Plasmid#132397Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1058APromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(Hygro) TagBFP-hNucleolin
Plasmid#182592Purposeexpresses bfp-hNucleolin in mammalian cellsDepositorInserthNucleolin (NCL Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgTelomere(F+E)
Plasmid#51024PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeresDepositorInsertOptimized sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_pre
Plasmid#119738PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA_pre
UseAAVTagsExpressionMammalianMutationPromoterhsyn1Available sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A)
Plasmid#221455PurposeAAV vector expressing GFAP-GeNLDepositorInsertGreen enhanced Nano-lantern
UseAAVTagsExpressionMutationPromoterhGFAPAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GpNLuc
Plasmid#208840PurposeExpresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-tubulin
Plasmid#136573PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670-tubulin
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-NavMs
Plasmid#100004PurposeElectrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFPDepositorInsertHis-NavMs, Voltage gated sodium channel
UseTagsHexa-His tag followed by a thrombin cleavage siteExpressionMammalianMutationC-terminus from a codon-optimized synthetic gene,…PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Luc2=tdT
Plasmid#32904PurposeExpresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.DepositorInsertsFirefly Luciferase
tandem Tomato Red Fluorescent Protein
UseTagstdTomato red fluorescent proteinExpressionMammalianMutationPromoterCMV-IE and n/aAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry
Plasmid#124494PurposeExpresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherryDepositorInsertFlipGFP(Caspase-1 cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEM-GECO1
Plasmid#32442PurposeMammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO1
UseTagsExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLQ-5S-F30-Mango IV
Plasmid#127584PurposeExpresses 5S-F30-Mango IV rRNA from a mU6 promoterDepositorInsert5S rRNA with F30 folding scaffold and Mango IV tag
UseTagsExpressionMammalianMutationPromotermodified U6Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/HA-FLAG-RBP2
Plasmid#14800DepositorInsertRetinoblastoma Binding Protein 2 (KDM5A Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FlipCherry-T2A-GFP
Plasmid#124436PurposeExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cellsDepositorInsertFlipCherry-T2A-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only