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Plasmid#171414PurposeFor the purifcation of human ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mTagBFP
Plasmid#105789PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mTagBFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1
Plasmid#111859PurposeFull length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, TEV site, and ZZExpressionInsectMutationPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-nTEVp
Plasmid#179666PurposeRas Binding Domain split-nTEVPDepositorInsertCRaf RBD protein (RAF1 Human)
UseTagsnTEVPExpressionMammalianMutationK65EPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-cTEVp
Plasmid#179667PurposeRas Binding Domain split-cTEVPDepositorInsertCRaf RBD protein (RAF1 Human)
UseTagscTEVPExpressionMammalianMutationK65EPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBB0323-TEV-His12
Plasmid#137039Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66700.1
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC - AVITEV
Plasmid#74059Purposeretroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1
Plasmid#83405Purposebacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-His-GvpC-TEV
Plasmid#153296PurposeExpresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertTEV-sensitive Gas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutation1) 21 bp TEV recognition and cleavage site flanke…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mVenus
Plasmid#105791PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mVenusExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-SMO-1(GG)
Plasmid#87054PurposeExpresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORFDepositorInsertsmo-1 (smo-1 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationThe last aa was removed to render processed SMO-1…PromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseC
Plasmid#89724PurposeExpresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage siteDepositorInsertPseC (pseC Campylobacter jejuni)
UseTagsHis8 and TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseH
Plasmid#89725PurposeExpresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage siteDepositorInsertPseH (pseH Campylobacter jejuni)
UseTags2xHis Gln 5xHis and TEVExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-TO-lyn-tvDiTEV
Plasmid#116061PurposeReporter plasmid for measuring protease activityDepositorInsertlyn-tvDiTEV
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQO18 TEV-L400C
Plasmid#27400DepositorInsertleuT
UseTagsHisX10 and TEVExpressionBacterialMutationChanged Leu 400 to CysPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-GST-TEV
Plasmid#223878Purpose4G-cloning donor for N-terminal His6-GST-TEV tagDepositorInsertHis6-GST-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-MBP-TEV
Plasmid#223877Purpose4G-cloning donor for N-terminal His6-MBP-TEV tagDepositorInsertHis6-MBP-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-HA2-TEV
Plasmid#223876Purpose4G-cloning donor for N-terminal His6-HA2-TEV tagDepositorInsertHis6-HA2-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3-TEV
Plasmid#223875Purpose4G-cloning donor for N-terminal His6-FLAG3-TEV tagDepositorInsertHis6-FLAG3-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only