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Plasmid#74258PurposeMammalian expression of N-terminal fusion of mRuby3 to H2B.DepositorInsertH2B
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Flex-mRuby3-2A
Plasmid#203846PurposeNeuron specific expression of a Cre-dependent mRuby3DepositorInsertmRuby3
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
UseTagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorInsertTubulin
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MTS-jAspSnFR3-mRuby3
Plasmid#203492PurposeMitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vectorDepositorInsertMTS-jAspSnFR3-mRuby3
UseLentiviralTagsHis-tag, Mitochondrial localization signal, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mRuby3-6xFLAG
Plasmid#161613PurposeCell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.DepositorInsertmRuby3-6xFLAG
UseTagsExpressionMammalianMutationN/APromoterHuman synapsin 1 promoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mRuby3-WPRE
Plasmid#193343PurposeAAV-mediated expression of mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-mRuby-Sec23A
Plasmid#36158DepositorInsertSec23A (SEC23A Human)
UseLentiviralTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Clover-Prx2-mRuby2
Plasmid#117906Purposefluorescent reporter of cytosolic hydrogen peroxideDepositorInsertClover-Prx2-mRuby2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab6b
Plasmid#166427PurposeRab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab6B
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 WT-mRuby3
Plasmid#179225Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mRuby2
Plasmid#185008PurposeFor knocking in FKBP F36V P2A mRuby2DepositorInsertFKBP F36V P2A mRuby2
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-actin
Plasmid#74255PurposeMammalian expression of N-terminal fusion of mRuby3 to actinDepositorInsertactin
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pHSE::Cre-mRuby3
Plasmid#164136PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat inductionDepositorInsertHSE promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterHSE synthetic promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME(S273A)-mRuby2-ST
Plasmid#109128PurposeModified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME(S273A)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Clover-Prx2-mRuby2
Plasmid#117907Purposefluorescent reporter of cytosolic hydrogen peroxideDepositorInsertClover-Prx2-mRuby2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10
Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-mRuby3-WPRE
Plasmid#107744PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-mRuby2
Plasmid#126215PurposeLabeling of cre-expressing neurons (red fluorescence)DepositorInsertmRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only